Relative expression of putative genes involved in galanthamine and other Amaryllidaceae alkaloids biosynthesis in Narcissus field and in vitro tissues

Unveiling Amaryllidaceae alkaloids: from biosynthesis to antiviral potential – a review

“…in AA biosynthesis with respect to its putative genes based on galanthamine was assessed by Aleya (2021). 57 The study indicates the expression level of NpN4OMT variation between the eld-grown bulb and basal tissues (signicantly higher) compared to in vitro cultures (bulblet and callus). Differential expression of NpN4OMT leads to alkaloid biosynthesis in later steps and thus to cellular differentiation.…”

Biotechnological Approaches to Optimize the Production of Amaryllidaceae Alkaloids

“…cultures [ 44 , 45 , 46 , 47 ], however, our molecular understanding regarding this complex biosynthesis route of AAs and its regulation is still unclear. Furthermore, the pattern of relative expression of putative AAs biosynthetic genes (in fields versus in vitro and in differentiated versus undifferentiated tissues of Narcissus development) added some clear knowledge regarding their role in alkaloid biosynthesis [ 89 ]. A study performed on callus culture of L. radiata showed how different factors, such as temperature (cold treatment), osmotic pressure (PEG treatment), or elicitor treatment (methyl jasmonate), can influence Lr OMT gene expression pattern [ 90 ].…”