Relative roles of mitochondrial and peroxisomal fatty acid oxidation in the metabolism of chylomicron remnants in rats and mice as assessed by a stable-isotope breath test

Martins, Ian; Vermeulen, Royce; Redgrave, Trevor G.

doi:10.1016/s0021-9150(99)00359-7

Cited by 8 publications

(11 citation statements)

References 18 publications

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“…Chylomicron-like emulsions rapidly incorporate the apolipoproteins A-I, A-II, A-IV, E, C-II and C-III from plasma, either by the release of apolipoproteins from plasma lipoproteins or by the association of apolipoproteins present free in plasma [114]. Results from recent studies show that remnant-like emulsion models are metabolized like CR [115][116], with remnant-like emulsions taken up by the liver, transported into endosomes and emulsion cholesteryl esters hydrolyzed in lysosomes. The fatty acids become available for oxidative metabolism, in particular metabolism to carbon dioxide [115][116][117].…”

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

“…Results from recent studies show that remnant-like emulsion models are metabolized like CR [115][116], with remnant-like emulsions taken up by the liver, transported into endosomes and emulsion cholesteryl esters hydrolyzed in lysosomes. The fatty acids become available for oxidative metabolism, in particular metabolism to carbon dioxide [115][116][117].…”

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

“…Breath tests are used for various clinical applications, such as the Helicobacter pylorii test [119], assessment of gastric emptying [120] and assessment of liver enyzmes [121]. 13 CO 2 and 14 CO 2 breath test for CR clearance and metabolism has been developed which is useful for non-invasive measurements of CR metabolism in mice, rats and rabbits [35,[115][116][117].…”

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

“…Apo E and C from the plasma associate with emulsion particles. After hydrolysis of emulsion triglyceride by LPL, remnant-like emulsions have been shown to mimic the metabolism of CR with oxidation of CR fatty acids by liver cells [35,[115][116][117]. Measurement by the breath test provide an assessment of the clearance and metabolism of the remnants of triglyeriderich lipoproteins and show the importance of apo E, LDL and LRP receptors in the clearance of CR in mice with genetic defects in lipoprotein metabolism Fig 2).…”

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

“…Measurement by the breath test provide an assessment of the clearance and metabolism of the remnants of triglyeriderich lipoproteins and show the importance of apo E, LDL and LRP receptors in the clearance of CR in mice with genetic defects in lipoprotein metabolism Fig 2). Further evaluation of the breath test has shown that the breath test reliably measures the metabolism of CR and that CR cholesteryl ester fatty acid is metabolized by mitochondrial pathways [116].…”

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

See 4 more Smart Citations

Obesity and post-prandial lipid metabolism. Feast or famine?

Martins

Redgrave

2004

The Journal of Nutritional Biochemistry

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Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

Section: Chylomicron and Cr-like Emulsions And Breath Testsmentioning

confidence: 99%

See 3 more Smart Citations

Obesity and post-prandial lipid metabolism. Feast or famine?

Martins

Redgrave

2004

The Journal of Nutritional Biochemistry

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Efflux of lipid from macrophages after induction of lipid accumulation by chylomicron remnants

Moore

Bejta

Avella

et al. 2005

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Oxidation of chylomicron remnant-like particles inhibits their uptake by THP-1 macrophages by apolipoprotein E-dependent processes

Bejta¹,

Moore²,

Avella³

et al. 2007

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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The influence of the oxidative state of chylomicron remnants (CMR) on the mechanisms of their uptake and induction of lipid accumulation by macrophages derived from the human monocyte cell line, THP-1, during foam cell formation was investigated using chylomicron-remnant-like particles (CRLPs) at 3 different levels of oxidation. The oxidative state of CRLPs was varied by exposure to CuSO4 (oxCRLPs) or incorporation of the antioxidant, probucol (pCRLPs) into the particles. oxCRLPs caused significantly less accumulation of triacylglycerol in the macrophages than CRLPs, and their rate of uptake was lower, while pCRLPs caused more lipid accumulation and were taken up faster. Uptake of all 3 types of particles was inhibited to a similar extent when entry via the low density lipoprotein (LDL) receptor related protein (80–90%), LDL receptor (− 30–40%), CD36 (− 40%) and phagocytosis (− 35–40%) was blocked using lactoferrin, excess LDL, anti-CD36 and cytochalasin D, respectively, but blocking scavenger receptors-A or -B1 using poly inosinic acid or excess HDL had no effect. These findings show that oxidation of CRLPs lowers their rate of uptake and induction of lipid accumulation in macrophages. However, oxidation does not change the main pathways of internalisation of CRLPs into THP-1 macrophages, which occur mainly via the LRP with some contribution from the LDLr, while CD36 and phagocytosis have only a minor role, regardless of the oxidative state of the particles. Thus, the effects of CMR oxidation on foam cell formation contrast sharply with those of LDL oxidation and this may be important in the role of dietary oxidized lipids and antioxidants in modulating atherosclerosis.

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Relative roles of mitochondrial and peroxisomal fatty acid oxidation in the metabolism of chylomicron remnants in rats and mice as assessed by a stable-isotope breath test

Cited by 8 publications

References 18 publications

Obesity and post-prandial lipid metabolism. Feast or famine?

Obesity and post-prandial lipid metabolism. Feast or famine?

Efflux of lipid from macrophages after induction of lipid accumulation by chylomicron remnants

Oxidation of chylomicron remnant-like particles inhibits their uptake by THP-1 macrophages by apolipoprotein E-dependent processes

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