Relaxant effect of the essential oil of Croton nepetifolius on ovine cervix

Abstract

Abstract:We investigated the in vitro effect of the essential oil of Croton nepetifolius Baill., Euphorbiaceae (EOCN), on spontaneous or induced contractions of circular and longitudinal muscles from the ovine cervix during the luteal phase of the estrous cycle. The relaxant effect of EOCN was expressed as a percentage of the contraction recorded before the addition of the oil and calculated relative to the preparations exposed only to the vehicle. The IC50 (concentration of oil required to produce a 50% maximal reduction in muscle contraction) for relaxation of spontaneous contractions in circular and longitudinal muscles was signifi cantly lower than the IC50 for blockade of K + -induced contraction (27.19 µg mL -1 versus 262.72 µg mL -1 and 40.92 µg mL -1 versus 222.47 µg mL -1 , respectively). Interestingly, there was a high degree of selectivity in the action of EOCN on cervix layers concerning the inhibition of acetylcholine-induced contraction in circular (IC50 277.10 µg mL -1 ) and longitudinal (IC50 52.56 µg mL -1 ) muscles. In conclusion, EOCN is able to relax ovine cervix during the luteal phase. This work opens the perspective of applying EOCN in ovine embryo transfer.

Essential Oils from Croton Species: Chemical Composition, in vitro and in silico Antileishmanial Evaluation, Antioxidant and Cytotoxicity Activities

“…Some examples are activity against human liver cancer cell lines, 5 anti-inflammatory, 6 antinociceptive, 7 hypoglycemic, antiulcer and antiestrogen. 8,9 The essential oils (EOs) of several Croton species (Euphorbiaceae) present insecticidal, 10 antifungal, 11 antileishmanial, 12 ovine cervix relaxant, 13 antibacterial and antioxidant 14 properties.…”

Use of natural antioxidants in in vitro mammalian embryo production

“…In general, extracts and essential oils are rich in phenolic compounds, carotenoids, terpenes, and other organic substances that have varied therapeutic properties (PEREIRA et al, 2012b) such as antioxidant activity (ESHTIYAGHI et al, 2016). Some of these compounds have also been studied in IVEP as antioxidants though they were not purified and of variable composition.…”

Efeito do óleo essencial do Croton nepetifolius Baill na penetração cervical em ovelhas mestiças de Dorper e Santa Inês

“…O Croton nepetifolius (Euforbiaceae) produz um óleo que exerce diversos efeitos sobre a musculatura lisa, tendo efeito relaxante e antiespasmódico (Pereira, 2006). Pereira et al (2012) avaliaram o efeito in vitro do óleo de Croton nepetifolius na musculatura cervical durante a fase luteal em cérvices ovinas oriundas de abatedouro e constataram uma inibição de 100% das contrações de toda musculatura cervical. Magalhães (2002) relatou que o OECn (óleo essencial de C. nepetifolius)promoveu relaxamento do músculo liso traqueal e detectou determinada especificidade desse efeito nessa musculatura.…”