Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

Mozgan, Eva-Maria; Edelmayer, Michael; Janjić, Klara; Pensch, Manuela; Fischer, Michael B.; Moritz, Andreas; Agis, Hermann

doi:10.1186/s12903-017-0357-6

Cited by 7 publications

(8 citation statements)

References 42 publications

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“…Both soft tissue wound-healing and bone regeneration are initiated by the formation of a fibrin clot. In the clots, activated platelets release a myriad of growth factors and cytokines/chemokines that stimulate the migration and proliferation of repair cells [ 20 , 21 ]. Among these factors are transforming growth factor-β1 (TGF-β1) and various isoforms of platelet-derived growth factor (PDGF).…”

Section: Introductionmentioning

confidence: 99%

Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices

et al. 2020

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Xenogeneic acellular collagen matrices represent a safe alternative to autologous soft tissue transplants in periodontology and implant dentistry. Here, we aimed to investigate the adsorption and release of growth factors from four porcine-derived collagen matrices using enzyme-linked immunosorbent assay. Non-crosslinked collagen matrix (NCM), crosslinked collagen matrix (CCM), dried acellular dermal matrix (DADM), and hydrated acellular dermal matrix (HADM) adsorbed each of the following growth factors, TGF-β1, FGF-2, PDGF-BB, GDF-5 and BMP-2, with an efficiency close to 100%. Growth factor release for a 13-day period was in the range of 10–50% of the adsorbed protein, except for the BMP-2 release that was in the range of 5–7%. Generally, protein release occurred in two phases. Phase I was arbitrary defined by the highest release from the matrices, usually within 24 h. Phase II, spanning the period immediately after the peak release until day 13, corresponded to the delayed release of the growth factors from the deeper layers of the matrices. HADM showed significantly (P < 0.001) higher TGF-β1, FGF-2, and PDGF-BB release in phase II, compared to the rest of the matrices. NCM exhibited significantly (P < 0.001) higher FGF-2 release in phase II, compared to CCM and DADM as well as a characteristic second peak in PDGF-BB release towards the middle of the tested period. In contrast to NCM and HADM, CCM and DADM showed a gradual and significantly higher release of GDF-5 in the second phase. Several burst releases of BMP-2 were characteristic for all matrices. The efficient adsorption and sustained protein release in the first 13 days, and the kinetics seen for HADM, with a burst release within hours and high amount of released growth factor within a secondary phase, may be beneficial for the long-term tissue regeneration following reconstructive periodontal surgery.

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Section: Introductionmentioning

confidence: 99%

Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices

et al. 2020

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“…We made the choice to coat the vCM with either enamel matrix derivative (EMD), which is widely used in periodontal regenerative surgery, or one of the following recombinant growth factors TGF-β1, PDGF-BB, FGF-2, or GDF-5 for a short, clinically relevant 10-min period. Our choice was partially based on the assumption that the majority of the listed proteins are released by activated platelets, and are thus naturally present in the blood clot [ 46 , 47 ]. Our results have shown that the vCM adsorbed each of the four growth factors with an efficiency higher than 93% ( Figure 2 a).…”

Section: Resultsmentioning

confidence: 99%

“…In this respect, our results of strongly induced SERPINE1 gene expression in primary hPDLCs, hOFs, and HUVECs grown on native as well as TGF-β1-, PDGF-BB- or FGF-2-coated vCMs strongly support the favorable effect of vCM on the regenerative process. The three growth factors TGF-β1, PDGF-BB, and FGF-2 as well as the investigated by us thrombospondin 1, encoded by the THBS1 gene in humans, are released by activated platelets at wounded sites [ 46 , 47 , 58 ]. All four factors are known to efficiently bind extracellular matrix proteins such as collagen [ 7 , 59 ] and thus, they may coat the vCM in situ after its placement at the defect site.…”

Section: Discussionmentioning

confidence: 99%

A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells

Asparuhova

Stähli

Guldener

et al. 2021

IJMS

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The aim of the present study was to investigate the influence of a novel volume-stable collagen matrix (vCM) on early wound healing events including cellular migration and adhesion, protein adsorption and release, and the dynamics of the hemostatic system. For this purpose, we utilized transwell migration and crystal violet adhesion assays, ELISAs for quantification of adsorbed and released from the matrix growth factors, and qRT-PCR for quantification of gene expression in cells grown on the matrix. Our results demonstrated that primary human oral fibroblasts, periodontal ligament, and endothelial cells exhibited increased migration toward vCM compared to control cells that migrated in the absence of the matrix. Cellular adhesive properties on vCM were significantly increased compared to controls. Growth factors TGF-β1, PDGF-BB, FGF-2, and GDF-5 were adsorbed on vCM with great efficiency and continuously delivered in the medium after an initial burst release within hours. We observed statistically significant upregulation of genes encoding the antifibrinolytic thrombomodulin, plasminogen activator inhibitor type 1, thrombospondin 1, and thromboplastin, as well as strong downregulation of genes encoding the profibrinolytic tissue plasminogen activator, urokinase-type plasminogen activator, its receptor, and the matrix metalloproteinase 14 in cells grown on vCM. As a general trend, the stimulatory effect of the vCM on the expression of antifibrinolytic genes was synergistically enhanced by TGF-β1, PDGF-BB, or FGF-2, whereas the strong inhibitory effect of the vCM on the expression of profibrinolytic genes was reversed by PDGF-BB, FGF-2, or GDF-5. Taken together, our data strongly support the effect of the novel vCM on fibrin clot stabilization and coagulation/fibrinolysis equilibrium, thus facilitating progression to the next stages of the soft tissue healing process.

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“…In a study by Mozgan E.M. et al [91], secretome of washed platelets (washed PSEC) and secretome of unwashed platelet preparations (unwashed PSEC) were lyophilized onto CBM. Supernatants were collected by adding and replacing medium after 1 to 48 h. Mitogenic response of human periodontal fibroblasts was induced by the supernatants and showed the highest levels of total protein, TGFβ1, and PDGF-BB in the first hour.…”

Section: Platelet Secretome (Psec)mentioning

confidence: 99%

Which substances loaded onto collagen scaffolds influence oral tissue regeneration?—an overview of the last 15 years

et al. 2020

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Background Collagen scaffolds are widely used for guided bone or tissue regeneration. Aiming to enhance their regenerative properties, studies have loaded various substances onto these scaffolds. This review aims to provide an overview of existing literature which conducted in vitro, in vivo, and clinical testing of drug-loaded collagen scaffolds and analyze their outcome of promoting oral regeneration. Materials and methods PubMed, Scopus, and Ovid Medline® were systematically searched for publications from 2005 to 2019. Journal articles assessing the effect of substances on oral hard or soft tissue regeneration, while using collagen carriers, were screened and qualitatively analyzed. Studies were grouped according to their used substance type—biological medical products, pharmaceuticals, and tissue-, cell-, and matrix-derived products. Results A total of 77 publications, applying 36 different substances, were included. Collagen scaffolds were demonstrating favorable adsorption behavior and release kinetics which could even be modified. BMP-2 was investigated most frequently, showing positive effects on oral tissue regeneration. BMP-9 showed comparable results at lower concentrations. Also, FGF2 enhanced bone and periodontal healing. Antibiotics improved the scaffold’s anti-microbial activity and reduced the penetrability for bacteria. Conclusion Growth factors showed promising results for oral tissue regeneration, while other substances were investigated less frequently. Found effects of investigated substances as well as adsorption and release properties of collagen scaffolds should be considered for further investigation. Clinical relevance: Collagen scaffolds are reliable carriers for any of the applied substances. BMP-2, BMP-9, and FGF2 showed enhanced bone and periodontal healing. Antibiotics improved anti-microbial properties of the scaffolds.

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Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

Cited by 7 publications

References 42 publications

Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices

Adsorption and Release of Growth Factors from Four Different Porcine-Derived Collagen Matrices

A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells

Which substances loaded onto collagen scaffolds influence oral tissue regeneration?—an overview of the last 15 years

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