Reliability of immunocytochemistry and fluorescence in situ hybridization on fine‐needle aspiration cytology samples of breast cancers: A comparative study

Ács, Balázs; Szekely, Nora; Szász, Attila Marcell; Lotz, Gábor; Szèkely, T.; Istók, Roland; Székely, Eszter; Madaras, Lilla; Kulka, Janina; Járay, Balázs

doi:10.1002/dc.23463

Cited by 13 publications

(6 citation statements)

References 28 publications

(34 reference statements)

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“…In our study, formalin-fixed cell block sections were used for breast biomarker testing. Previous studies suggested that IHCs for HER2, ER and PR performed on FFPE cell blocks were reliable in predicting the expression of these markers [18,19,20,21,22]. In our study, however, the overall change rate for ER was significantly higher in the cytological specimens than in the surgical specimens.…”

Section: Discussioncontrasting

confidence: 55%

Correlation of Expression of Breast Biomarkers in Primary and Metastatic Breast Carcinomas: A Single-Institution Experience

Hou

Shen²,

Chaudhary³

et al. 2016

Acta Cytologica

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Objective: Changes in the status of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) in metastatic breast carcinomas are frequently reported. We examined the change in the status of biomarkers in metastatic breast carcinomas. Study Design: This study cohort was composed of 137 metastatic breast carcinomas (58 surgical and 79 cytological specimens) with existing primary tumors during a study period from 2013 to 2015. Results: The overall change rates in metastases were 9, 21 and 6% for ER, PR and HER2, respectively. All changes were from positive in the primary tumor to negative in the metastases. The ER change rate was significantly higher in the cytological than in the surgical metastases. Six of 14 cytological metastases with positive HER2 in primary tumors showed a change in HER2 status, including 5 fluid specimens and 1 fine-needle aspiration (FNA); the other 8 had no change in HER2 status, and included 7 FNAs and 1 fluid specimen. Conclusion: The significant percentage of cases with a change in biomarker status supports the recommendation by the College of American Pathologists to test breast biomarkers in metastases. HER2 status change was mostly identified in fluid specimens; however, the small sample size in our cohort and the fact that HER2 fluorescence in situ hybridization was not performed may warrant further studies.

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Section: Discussioncontrasting

confidence: 55%

Correlation of Expression of Breast Biomarkers in Primary and Metastatic Breast Carcinomas: A Single-Institution Experience

Hou

Shen²,

Chaudhary³

et al. 2016

Acta Cytologica

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“…-mammary cells-for the diagnosis of breast cancer [4]; -cervical cells-for the diagnosis of concomitant diseases [5] [6];…”

Section: Introductionmentioning

confidence: 99%

Using of Color Model and Contrast Variation in Wavelet Ideology for Study <i>Megaloblastic Anemia Cells</i>

Lyashenko¹,

Babker²

2017

OJBD

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Processing and analyzing of medical images is one of the priority research areas. At the same time, the processing of images of cells occupies a special place. This is due to the fact that such studies allow for a comprehensive diagnosis of the state of human health, identify and prevent the development of diseases in the early stages. We investigate the effectiveness of using wavelet analysis in color models, taking into account the preliminary change in the contrast of the input image. We consider the HSV color model and the image contrast modification procedure, which is based on the histogram change in the specified range with gamma correction. As a criterion for choosing parameters for changing the contrast of the image, we consider the entropy of the image. We also showed the advisability of using the value of the entropy index for the subsequent improvement of image analysis based on the wavelet ideology. We examined the general sequence of action for the analysis of image of megaloblastic anemia cells. This sequence is based on the choice of parameters for changing the contrast of the image and application of wavelet ideology.

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“…1 First of all, reliability (precision) and validity (accuracy) are two completely different methodological issues which should not be confused. They aimed to assess the diagnostic accuracy of ancillary tests applied to fine-needle aspiration cytology (FNAB) in breast tumors and metastases, as a favorable first choice.…”

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confidence: 99%

“…The authors compared ER and Her2 immunocytochemical (ICC) as well as immunohistochemical (IHC) staining and HER2 fluorescence in situ hybridization (FISH). 1 First of all, reliability (precision) and validity (accuracy) are two completely different methodological issues which should not be confused. (Title of the paper and the aim of the authors indicate to this confusion).…”

mentioning

confidence: 99%

“…They reported that significant difference (P < 0.001) and moderate correlation (j 5 0.44) were noted between results of 97 paired ICC an IHC reactions for ER expression. 1 Considering large sample size, statistically significant (P value < 0.001) differences can easily be obtained; however, clinically importance of the observed difference should be taking into account instead of statistically significant. Moreover, for qualitative variables, weighted kappa should be used.…”

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confidence: 99%

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Reliability of immunocytochemistry and fluorescence in situ hybridization on fine‐needle aspiration cytology samples of breast cancers: methodological issues

Sabour

2016

Diagnostic Cytopathology

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I was interested to read the paper by Acs B and colleagues published in the Jun 2016 issue of Diagn Cytopathol. They aimed to assess the diagnostic accuracy of ancillary tests applied to fine-needle aspiration cytology (FNAB) in breast tumors and metastases, as a favorable first choice. The authors compared ER and Her2 immunocytochemical (ICC) as well as immunohistochemical (IHC) staining and HER2 fluorescence in situ hybridization (FISH). 1 First of all, reliability (precision) and validity (accuracy) are two completely different methodological issues which should not be confused. (Title of the paper and the aim of the authors indicate to this confusion). They reported that significant difference (P < 0.001) and moderate correlation (j 5 0.44) were noted between results of 97 paired ICC an IHC reactions for ER expression. 1 Considering large sample size, statistically significant (P value < 0.001) differences can easily be obtained; however, clinically importance of the observed difference should be taking into account instead of statistically significant. Moreover, for qualitative variables, weighted kappa should be used. It is crucial to know that simple kappa has two important limitations as below. It depends on the prevalence of each cell (concordant and discordant cells) as well as the number of the categories (the higher the categories, the lower our kappa value. [2][3][4][5][6][7][8] As the authors pointed out in their conclusion, FISH performed on FNAB samples is suitable to categorize primary and metastatic breast cancer in regard of HER2 gene amplification status and can be used as a predictive test in respect of therapies targeting HER2. For prediction, we need two cohort dataset otherwise, we should split one cohort dataset to develop and validate our model. It means without validation, we cannot generalize our results. 2-8 Therefore, such a conclusion can be a misleading message and misdiagnosis as well as mismanagement of the patients cannot be avoided. References 1. Acs B, Szekely N, Szasz AM et al. Reliability of immunocytochemistry and fluorescence in situ hybridization on fine-needle aspiration cytology samples of breast cancers: A comparative study.

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Reliability of immunocytochemistry and fluorescence in situ hybridization on fine‐needle aspiration cytology samples of breast cancers: A comparative study

Cited by 13 publications

References 28 publications

Correlation of Expression of Breast Biomarkers in Primary and Metastatic Breast Carcinomas: A Single-Institution Experience

Correlation of Expression of Breast Biomarkers in Primary and Metastatic Breast Carcinomas: A Single-Institution Experience

Using of Color Model and Contrast Variation in Wavelet Ideology for Study <i>Megaloblastic Anemia Cells</i>

Reliability of immunocytochemistry and fluorescence in situ hybridization on fine‐needle aspiration cytology samples of breast cancers: methodological issues

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Reliability of immunocytochemistry and fluorescence in situ hybridization on fine‐needle aspiration cytology samples of breast cancers: A comparative study

Cited by 13 publications

References 28 publications

Correlation of Expression of Breast Biomarkers in Primary and Metastatic Breast Carcinomas: A Single-Institution Experience

Correlation of Expression of Breast Biomarkers in Primary and Metastatic Breast Carcinomas: A Single-Institution Experience

Using of Color Model and Contrast Variation in Wavelet Ideology for Study &lt;i&gt;Megaloblastic Anemia Cells&lt;/i&gt;

Reliability of immunocytochemistry and fluorescence in situ hybridization on fine‐needle aspiration cytology samples of breast cancers: methodological issues

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Using of Color Model and Contrast Variation in Wavelet Ideology for Study <i>Megaloblastic Anemia Cells</i>