Reliable multiplex generation of pooled induced pluripotent stem cells

Smullen, Molly; Olson, Meagan N.; Reichert, Julia M.; Dawes, Pepper; Murray, Liam F.; Baer, Christina E.; Wang, Qi; Readhead, Benjamin; Church, George M.; Lim, Elaine T.; Chan, Yingleong

doi:10.1016/j.crmeth.2023.100570

Cell Reports Methods

2023

DOI: 10.1016/j.crmeth.2023.100570

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Reliable multiplex generation of pooled induced pluripotent stem cells

Molly Smullen,

Meagan N. Olson,

Julia M. Reichert

et al.

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2024

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Enriching iPSC research diversity: Harnessing human biobank collections for improved ethnic representation

Gurwitz,

Steeg

2024

Drug Development Research

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Biobanks of human biosamples and cell lines are indispensable for biomedical research on human health and disease and for drug development projects. Many human cell line biobanks worldwide hold collections of lymphoblastoid cell lines (LCLs), representing thousands of affected and control donors from diverse ethnic/ancestry groups. In recent years, induced human pluripotent stem cells (iPSCs) and differentiated human cells derived from these iPSCs have become indispensable for applied biomedical research. Establishing iPSCs remains a laborious and costly step towards generating differentiated human cells. To address this research need, several non‐profit and commercial biobanks have established iPSC collections for distribution to researchers, thereby serving as a resource for generating differentiated human cells. The most common starting materials for generation of iPSCs are a skin biopsy for harvesting fibroblasts, or a blood sample for collection of peripheral blood mononuclear cells. However untapped resources include the large established collections of biobanked human LCLs which can be reprogrammed to iPSCs using a variety of published protocols including the use of non‐integrating episomal vectors. Many biobanks curate LCLs from diverse ethnic/ancestry populations, an aspect largely absent in most established iPSC biobanks which tend to primarily reflect populations from developed countries. Here, we call upon researchers across the breadth of iPSC research to tap the unique resource of existing and diverse human LCL collections for establishing biobanked iPSC panels that better represent the varied human ethnic (and hence genomic) diversity, thereby benefiting precision medicine and drug development research on a global scale.

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Enriching iPSC research diversity: Harnessing human biobank collections for improved ethnic representation

Gurwitz,

Steeg

2024

Drug Development Research

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show abstract

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Reliable multiplex generation of pooled induced pluripotent stem cells

Cited by 1 publication

References 56 publications

Enriching iPSC research diversity: Harnessing human biobank collections for improved ethnic representation

Enriching iPSC research diversity: Harnessing human biobank collections for improved ethnic representation

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