This study delves into the profound impact and significance of religious culture on the urban spatial configuration and local cultural essence of Macau amidst contemporary societal transformations. Emphasis is placed on how religious sites undergo functional reshaping and role redefinition within the urban fabric, thereby influencing residents’ quality of life, cityscape, and cultural identity. Adopting a spatial anthropological lens, this research employs a multitude of methodologies including city walking, participatory observation, and historical analysis to investigate the spatial configurations and developmental trajectories of Macau’s diverse religious sites and their immediate environments. The aim is to unearth the historical drivers behind the formation and dissemination of religious culture in Macau and to elucidate its intricate interactions with urban space. The findings reveal that religious sites in Macau not only physically delineate unique urban spatial patterns but also serve as vital hubs for fostering local cultural cohesion and weaving social networks through routine religious practices, community engagements, and a plethora of festive and ritualistic events. The pervasive influence of these sites extends across multiple domains of society, culture, and economy, playing a pivotal role in maintaining regional stability and harmony, enriching cultural diversity, and propelling socioeconomic transformation.