Remission induction chemotherapy induces in vivo caspase‐dependent apoptosis in bone marrow acute myeloid leukemia blast cells and spares lymphocytes

Vial, Jean; Tabrizi, Reza; Pigneux, Arnaud; Lacombe, Francis; Perreten, Vincent; Belloc, Françis

doi:10.1002/cyto.b.20105

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…One study of adult AML patients suggested that cytarabine and daunorubicin therapy specifically depleted blast cells but left the bone marrow lymphocytes intact 36 . Therefore, this study provides an important perspective on the immunologic effects of AML chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

Immunologic Consequences of Chemotherapy for Acute Myeloid Leukemia

Reilly

Kersun²,

Prak³

et al. 2013

Journal of Pediatric Hematology/Oncology

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There are few data characterizing the immunologic consequences of chemotherapy for AML and almost nothing is known about the effects of chemotherapy in a pediatric AML cohort. We identified T cell subsets, B cell subsets and used ELISPOT analyses to define the function of T cells and B cells in seven pediatric patients with AML on chemotherapy. The data demonstrate that the effects of chemotherapy disproportionately target the B cell and depletion of B cells is associated with impaired responses to the inactivated influenza vaccine. Diminished T cell numbers were also observed although the magnitude of the effect was less than what was seen for B cells. Furthermore, measures of T cell function were largely intact. We conclude that humoral immunity is significantly affected by chemotherapy for AML.

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Section: Discussionmentioning

confidence: 99%

Immunologic Consequences of Chemotherapy for Acute Myeloid Leukemia

Reilly

Kersun²,

Prak³

et al. 2013

Journal of Pediatric Hematology/Oncology

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“…On leukemia samples, blast cells and lymphocytes were identified by flow cytometry on the basis of their scatter properties and CD45 (PC5-conjugated anti-CD45; Beckman-Coulter, Margency, France) expression [23,24]. On normal bone marrow mononuclear cells, progenitor cells were identified on the basis of CD34 expression and scatter properties.…”

Section: Analysis Of Apoptosis By Flow Cytometrymentioning

confidence: 99%

Triptolide cooperates with chemotherapy to induce apoptosis in acute myeloid leukemia cells

Pigneux

Mahon

Uhalde

et al. 2008

Experimental Hematology

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“…He underlined the importance of cardiolipin (CL) as a key point in apoptosis pathway (88)(89)(90)(91)(92). CL is a mitochondria-specific phospholipid intimately involved in several apoptotic steps.…”

Section: Cell Death Drug Testing and Toxicologymentioning

confidence: 99%

Cytomics and nanobioengineering

Pierzchalski¹,

Robitzki²,

Mittag³

et al. 2008

Cytometry Part B Clinical

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The finding that an individual's genome differs as much as by many million variants from that of the human reference assembly diminished the great enthusiasm that every disease could be predicted based on nucleotide polymorphisms. Even individual cells of an organ may be specifically equipped to perform specific tasks and that the information of individual cells in a cell system is key information to understand function or dysfunction. Therefore, cytomics received great attention during the last years as it allows to quantitatively and qualitatively analyzing great number of individual cells, cell constituents, and of their intracellular and functional interactions in a cellular system and also giving the concept of analysis of these data.Exhaustive data extraction from multiparametric assays and multiple tests are the prerequisite for prediction of drug toxicity. Cytomics, as novel approach for unsupervised data analysis give a chance to find the most predictive parameters, which describe best the toxicity of a chemical. Cytomics is intrinsically connected to drug development and drug discovery.Focused on small structures, nanobioengineering is the ideal partner of cytomics, the systems biological discipline for cell population analysis. Realizing the idea ''from the molecule to the patient'' develops and offers chemical compounds, proteins, and other biomolecules, cells as well as tissues as instruments and products for a wide variety of biotechnological and biomedical applications.The integrative nanobioengineering combining different disciplines of nanotechnology will promote the development of innovative therapies and diagnostic methods. It can improve the precision of the measurements with focus on single cell analysis. By nanobioengineering and whole body imaging techniques, cytomics covers the field from molecules through bacterial cells, eukaryotic tissues, and organs to small animal live analysis. Toxicological testing and medical drug development are currently strongly broadening. It harbors the promise to substantially impact on various fields of biomedicine, drug discovery, and predictive medicine.As the number of scientific data is rising exponentially, new data analysis tools and strategies like cytomics and nanobioengineering take a lead and get closer to application. Bionanoengineering may strongly support the quantitative data supply, thus strengthening the rationale for cytomics approach. q

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Remission induction chemotherapy induces in vivo caspase‐dependent apoptosis in bone marrow acute myeloid leukemia blast cells and spares lymphocytes

Cited by 3 publications

References 28 publications

Immunologic Consequences of Chemotherapy for Acute Myeloid Leukemia

Immunologic Consequences of Chemotherapy for Acute Myeloid Leukemia

Triptolide cooperates with chemotherapy to induce apoptosis in acute myeloid leukemia cells

Cytomics and nanobioengineering

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