Remote focusing in a temporal focusing microscope

Abstract

In a temporal focusing microscope, dispersion can remotely shift the temporal focal plane axially, but only a single depth can be in focus at a time on a fixed camera. In this paper, we demonstrate remote focusing in a temporal focusing microscope. Dispersion tuning with an electrically tunable lens (ETL) in a 4f pulse shaper scans the excitation plane axially, and another ETL in the detection path keeps the shifted excitation plane in focus on the camera. Image stacks formed using two ETLs versus a traditional stage scan are equivalent.

Three-photon excited fluorescence imaging in neuroscience: From principles to applications

“…Remote focusing is an optical refocusing technique that is implemented remotely from the specimen ( Anselmi et al, 2011 ; Thériault et al, 2014 ; Cheng Z. et al, 2020 ; Jiang et al, 2020 ; Durst et al, 2021 ). As shown in Figure 4E , this module typically uses a remote objective and a movable lightweight mirror to refocus the imaging plane within the specimen ( Schuetzenberger and Borst, 2020 ).…”