Remote post-conditioning and allopurinol reduce ischemia-reperfusion injury in an infra-renal ischemia model

Brandão, Rafael Inácio; Gomes, Ricardo Zanetti; Lopes, Luana Pontes Barros; Linhares, Filipe; Vellosa, José Carlos Rebuglio; Paludo, Kátia Sabrina

doi:10.1177/1753944718803309

Cited by 7 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The literature reviewed showed that several authors have reported results in agreement with those described here. They conclude that when allopurinol is administered, the effect on the prevention of IRI is only significant in those cases in which this treatment is associated with ischemic postconditioning, that is, periods of arterial reocclusion of a few minutes duration applied at the beginning of tissue reperfusion and which have shown a decrease in the damage associated with ROS [31,32]. In contrast, there are also studies in which its oral administration prior to ischemia instauration does demonstrate an antioxidant effect, as evidenced by a reduction in HID and a decrease in the production of oxidative stress markers such as malondialdehyde (MDA) [33].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats

et al. 2021

View full text Add to dashboard Cite

Intestinal ischemia-reperfusion injury (i-IRI) is a rare disorder with a high mortality rate, resulting from the loss of blood flow to an intestinal segment. Most of the damage is triggered by the restoration of flow and the arrival of cytokines and reactive oxygen species (ROS), among others. Inactivation of these molecules before tissue reperfusion could reduce intestinal damage. The aim of this work was to analyze the preventive effect of allopurinol and nitroindazole on intestinal mucosal damage after i-IRI. Wag/RijHsd rats were subjected to i-IRI by clamping the superior mesenteric artery (for 1 or 2 h) followed by a 30 min period of reperfusion. Histopathological intestinal damage (HID) was assessed by microscopic examination of histological sections obtained from injured intestine. HID was increased by almost 20% by doubling the ischemia time (from 1 to 2 h). Nitroindazole reduced HID in both the 1 and 2 h period of ischemia by approximately 30% and 60%, respectively (p < 0.001). Our preliminary results demonstrate that nitroindazole has a preventive/protective effect against tissue damage in the early stages of i-IRI. However, to better understand the molecular mechanisms underlying this phenomenon, further studies are needed.

show abstract

Section: Discussionmentioning

confidence: 99%

Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Gholampour et al treated [37] with remote ischemic perconditioning and quercetin which reduced plasma AST activity in the liver after renal I/R. Brandão et al observed [38] separately the protective effects of allopurinol and the benefits of ischemic post-conditioning by measuring ASTl with significantly reduced I/R systemic injuries in rats.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Hypertransferasemic Effects of Erythropoietin and U-74389G on Aspartate Aminotransferase Levels

Tsompos,

Panoulis,

Toutouzas

et al. 2018

Clin Res Hematol

View full text Add to dashboard Cite

Aim: This study calculated the effects on aspartate aminotransferase (AST) levels, after treatment with either of two drugs: The erythropoietin (Epo) and the antioxidant lazaroid (L) drug U-74389G. The calculation was based on the results of two preliminary studies, each one of which estimated the certain influence, after the respective drug usage in an induced ischemiareperfusion (IR) animal experiment. Materials and Methods: The two main experimental endpoints at which the serum AST levels were evaluated were the 60 th reperfusion min (for the Groups A, C, and E) and the 120 th reperfusion min (for the Groups B, D, and F). Especially, Groups A and B were processed without drugs, Groups C and D after Epo administration, whereas Groups E and F after the L administration. Results: The first preliminary study of Epo presented a significant hypertransferasemic effect by 19.73 ± 7.71% (P = 0.0119). The second preliminary study of U-74389G presented a nonsignificant hypertransferasemic effect by 16.23 ± 8.59% (P = 0.0583). These two studies were coevaluated since they came from the same experimental setting. The outcome of the coevaluation was that L is 0.8224656-fold (0.8211631-0.8237701) less hypertransferasemic than Epo (P = 0.0000). Conclusions: The antioxidant capacities of U-74389G ascribe 0.8224656-fold less hypertransferasemic effects than Epo (P = 0.0000).

show abstract

“…"Ischemia" is defined as the loss of blood flow by depriving a tissue of metabolic substrates such as glucose as a result of reduced blood flow and impaired nutrition. "Reperfusion" is used to describe the restoration of blood flow and tissue nutrition after a period of ischemia and is a critical step in dealing with any ischemia condition 1,2 . Ischemia is associated with progressive decline in cellular functions, increased oxidative and inflammatory response.…”

Section: Introductionmentioning

confidence: 99%

Changes in cardiac cells due to ticagrelor and enoxaparin in a rat ischemia/reperfusion model

Findik

Barış

Yazır

et al. 2021

Rev. Assoc. Med. Bras.

View full text Add to dashboard Cite

OBJECTIVE: Studies on ischemia/reperfusion injury remain the focus of interest. Ticagrelor and enoxaparin, which are antiaggregant and anticoagulant drugs developed for use in many cardiovascular pathologies, are still included in many ischemia/reperfusion studies.Remarkably, their new protective effects, especially with regard to ticagrelor, continue to be reported in the current literature. The aim of this study was to evaluate the beneficial effects of ticagrelor and enoxaparin pretreatments on the rat heart with histological and immunohistochemical markers in an ischemia/reperfusion model. METHODS:Wistar-albino rats (weighing 350-400 g) were divided into four groups as follows: Sham-Control (Group 1), Control-Saline+ischemia/reperfusion (Group 2), Ticagrelor+ischemia/reperfusion (Group 3), and Enoxaparin+ischemia/reperfusion (Group 4). The ischemia/reperfusion injury model was applied to Group 2, Group 3 and Group 4. Heart tissue sections were stained with hematoxylin and eosin for histological examinations. Caspase 3 immunostaining was evaluated to detect apoptosis in the heart tissue sections. RESULTS:Both pretreatments ameliorated the ischemic damage but especially tissue sections belonging to Group 3 were nearly similar to control levels. The results indicated that ischemia/reperfusion-induced myocardial damage was significantly increased in Group 2, whereas ticagrelor and enoxaparin pretreatments in Group 3 and Group 4 significantly decreased apoptotic scores and the histological appearance of the Group 3 close to the normal myocardium (p<0.001).CONCLUSION: As supported by histological findings in our study, ticagrelor and enoxaparin have protective properties for heart tissue in this ischemia/reperfusion injury model.

show abstract

Remote post-conditioning and allopurinol reduce ischemia-reperfusion injury in an infra-renal ischemia model

Cited by 7 publications

References 27 publications

Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats

Antioxidant-Based Therapy Reduces Early-Stage Intestinal Ischemia-Reperfusion Injury in Rats

Comparison of the Hypertransferasemic Effects of Erythropoietin and U-74389G on Aspartate Aminotransferase Levels

Changes in cardiac cells due to ticagrelor and enoxaparin in a rat ischemia/reperfusion model

Contact Info

Product

Resources

About