REMOVED: In vitro propagation and assessment of genetic fidelity in Dioscorea deltoidea, a potent diosgenin yielding endangered plant

Nazir, Romaan; Gupta, Suphla; Dey, Abhijit; Kumar, Vijay; Yousuf, Muzamil; Hussain, Shahnawaz; Dwivedi, Padmanabh; Pandey, Devendra Kumar

doi:10.1016/j.sajb.2020.07.018

Cited by 25 publications

(5 citation statements)

References 48 publications

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“…Diosgenin, a triterpene and aglycone derivative of dioscin has been recorded as the most popular phytosteroid sapogenin from the members of the Dioscorea species. Diosgenin has been known to exhibit potent anticancer, antidiabetic, antithrombosis, anticoagulant, anti‐inflammatory, antiaging, and antiviral agents and a number of biotechnological interventions were carried out to optimize diosgenin production from various botanicals 163,164 . Effects of extraction efficacy, organ selection, drying method, and seasonal variation were also analyzed during HPTLC quantification of diosgenin from Dioscorea deltoidea 165 .…”

Section: Diosgenin Versus Dioscinmentioning

confidence: 99%

“…Diosgenin has been known to exhibit potent anticancer, antidiabetic, antithrombosis, anticoagulant, anti-inflammatory, antiaging, and antiviral agents and a number of biotechnological interventions were carried out to optimize diosgenin production from various botanicals. 163,164 Effects of extraction efficacy, organ selection, drying method, and seasonal variation were also analyzed during HPTLC quantification of diosgenin from Dioscorea deltoidea. 165 In addition, endophytic sources of diosgenin and phosphate solubilizing bacteria from the rhizosphere of Dioscorea alata promoting diosgenin yield were also evaluated.…”

Section: Diosgenin Versus Dioscinmentioning

confidence: 99%

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Dioscin: A review on pharmacological properties and therapeutic values

et al. 2021

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Dioscin has gained immense popularity as a natural, bioactive steroid saponin, which offers numerous medical benefits. The growing global incidence of disease-associated morbidity and mortality continues to compromise human health, facilitating an increasingly urgent need for nontoxic, noninvasive, and efficient treatment alternatives. Natural compounds can contribute vastly to this field. Over recent years, studies have demonstrated the remarkable protective actions of dioscin against a variety of human malignancies, metabolic

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Section: Diosgenin Versus Dioscinmentioning

confidence: 99%

Section: Diosgenin Versus Dioscinmentioning

confidence: 99%

Dioscin: A review on pharmacological properties and therapeutic values

et al. 2021

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“…An appropriate amount of methanol was added to the residue and nal concentration was ltered through 0.2 µM syringe lter and preserved in refrigerator (4 °C) for further analysis. [50] GC-MS analysis An Agilent 7890A gas chromatography coupled to a 5875C mass spectrometer detector (XL MSD) with triple axis and mass hunter work station software (USA) was used for the analysis of diosgenin. Chromatography was performed on DB-5: 30 m x 0.25 mm i.d.…”

Section: Sample Preparationmentioning

confidence: 99%

Enhanced in vitro production of diosgenin in shoot cultures of Dioscorea deltoidea by elicitation and precursor feeding

Nazir

Gupta

Kumar

et al. 2020

Preprint

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Background: Dioscorea deltoidea (Family: Dioscoreaceae) is a critically endangered medicinal plant widely used in traditional medicine and pharmaceutical industries for the preparation of appropriate drugs. The present study was conducted to investigate the effect of different elicitors such as salicylic acid (SA), methyl jasmonate (MeJa), jasmonic acid (JA) and abscisic acid (ABA) on the synthesis of diosgenin production in D. deltoidea shoot cultures. In addition, the effect of different precursors (squalene, β-sitosterol, and cholesterol) was also demonstrated. Results: Results showed that precursors significantly influenced diosgenin production as compared to elicitors. Application of SA (200 µM) showed highest diosgenin production (0.912% DW) following 4 h of incubation time, whereas MeJA (100 µM) exhibited 0.814% DW of diosgenin content following 8 h of incubation time. Among precursors, β-sitosterol at 200 µM produced maximum diosgenin content (1.006% DW) followed by 100 µM squalene (0.947% DW) harvested after 5th day of the precursor addition. Interestingly, cholesterol showed low diosgenin production, but significant than control cultures. Finally response surface methodology (RSM) was conducted to see the interactive effect of best elicitors and precursors concentration for better yield of diosgenin and biomass.Conclusions: The results revealed that exposure to different elicitors and precursors have a significant influence on accumulation of diosgenin in D. deltoidea shoot cultures. The optimized conditions for SA and β-sitosterol for the better yield of diosgenin and biomass were found to be 165 µM, 150 µM and 170 µM, 140 µM respectively.

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“…PGRs are another important parameter for in vitro plant regeneration. Auxin and cytokinin have been used for induction of regeneration in Dioscorea deltoidea and an efficient system from nodal segments of pharmaceutically important plant D. deltoidea has been established 18 . However, most papers mainly focused on the screening and optimization of in vitro culture conditions of single kind of explant 19 – 22 ; few studied different explants and mechanism of in vitro morphogenesis in D. nipponica .…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino

Dang

Gao

Zhang

et al. 2022

Sci Rep

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Dioscorea nipponica Makino is an optimal candidate to develop the diosgenin industry in North China. Due to its increasing demand in the medicine industry, it is urgent to apply new biotechnological tools to foster breeds with desirable traits and enhanced secondary metabolite production. The production of useful metabolites by the in vitro cultured rhizomes can be explored successfully for utilization by various food and drug industries. In this study, we reported callus formation and plantlet regeneration of the medicinal plant D. nipponica. Explants of leaves, stem segments and rhizomes of aseptic seedlings were cultured on Murashige and Skoog (MS) medium containing various combinations of auxin and cytokinin to find the optimal PGRs of each type of explant for callus induction and shoot regeneration of D. nipponica. The paraffin section technique was also used to observe of the morphogenesis of callus and adventitious bud. Explants of seeds and rhizomes formed calli at high frequency in all lines we examined. However, the explant of leaves rarely formed callus. Three kinds of callus were detected during the induction phase. Here, we describe three types of callus (Callus I–III) with different structure characteristics. Greenish in color and a nodule-like protrusion surface (Callus type III) were arranged more closely of cells with less interstitial substance, cell differentiation ability stronger than other callus types. The optimum combination was the maximum shoot differentiation frequency of 90% in callus derived from seeds cultured on MS medium with 2.0 mg L−16-BA + 0.2 mg L−1NAA. The shoot differentiation frequency (88.57%) of rhizome-induced callus was obtained by the combination of MS medium supplemented with 3.0 mg L−16-BA + 2.0 mg L−1NAA. 1/2 MS medium plus 0.5 mg L−1NAA resulted in a higher root regeneration frequency of 86.70%. In vitro propagated plantlets with healthy roots were domesticated and transplanted into small plastic pots containing sterile soil rite under greenhouse conditions with 80% survivability. Bud differentiation is mostly of exogenous origin, mostly occurring on the near callus surface. Therefore, it may be surmised that in vitro morphogenesis of D. nipponica is mainly caused by indirect organogenesis (adventitious bud).

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REMOVED: In vitro propagation and assessment of genetic fidelity in Dioscorea deltoidea, a potent diosgenin yielding endangered plant

Cited by 25 publications

References 48 publications

Dioscin: A review on pharmacological properties and therapeutic values

Dioscin: A review on pharmacological properties and therapeutic values

Enhanced in vitro production of diosgenin in shoot cultures of Dioscorea deltoidea by elicitation and precursor feeding

In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino

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