RENEB biodosimetry intercomparison analyzing translocations by FISH

Barquinero, Joan Francesc; Beinke, Christina; Borràs, Mireia; Buraczewska, Iwona; Darroudi, F.; Grégoire, Éric; Hristova, Rositsa; Kulka, Ulrike; Lindholm, Carita; Moreno, M. Echevarría; Moquet, Jayne; Oestreicher, Ursula; Prieto, Manuel Ángel Rodríguez; Pujol, Mònica; Ricoul, Michelle; Sabatier, Laure; Sommer, Sylwester; Sun, Mingzhu; Wójcik, Andrzej; Barrios, Leonardo

doi:10.1080/09553002.2016.1222092

Cited by 24 publications

(21 citation statements)

References 27 publications

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“…Key to the Bayesian concept is the application of the inversion theorem in its continious version, i.e. : (4) where D denotes the unknown parameter (absorbed dose) and X obs the observation (the dicentric yield within the sample, y, and the calibration data). Thus, the posterior dose distribution (or calibrative dose density), P(D|X obs ), scales with the product of the likelihood (or predictive density) P(X obs |D) and the prior P(D):…”

Section: Probabilistic Approach: Bayesian Methodsmentioning

confidence: 99%

“…DCA, PCC and CBMN show stability of responses for several months, as induction of these aberrations means the cells cannot reproduce and thus the cells die and these aberrations are gradually removed from the population of circulating lymphocytes, Therefore, the relative specificity to ionising radiation drives the accuracy of these methods. In contrast, γ-H2AX and FISH are not radiation specific, however, the individual background levels are becoming more well understood 4,6,32 . The vast majority of γ-H2AX aberrations disappear within approximately 24 hours and thus this is very much a short term assay, however, translocations detected by FISH are stable over many years.…”

Section: Biodosimetrymentioning

confidence: 99%

“…The cytokinesis-blocked micronucleus (CBMN) assay is also regarded as an important tool for biological assessment of radiation dose and is growing in importance 3 . For longer-term retrospective dosimetry, fluorescence in situ hybridization (FISH) staining of translocations can be used 4 . In addition, new techniques for premature chromosome condensation and scoring offer alternative methodologies 5 and the γ-H2AX assay allows direct measurement of double-strand breaks (DSB), which are caused almost exclusively by ionising radiation, in a human cell 6 .…”

Section: Introductionmentioning

confidence: 99%

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Uncertainty on Radiation Doses Estimated by Biological and Retrospective Physical Methods

Ainsbury

Samaga

Monaca

et al. 2017

Radiation Protection Dosimetry

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Biological and physical retrospective dosimetry are recognised as key techniques to provide individual estimates of dose following unplanned exposures to ionising radiation. Whilst there has been a relatively large amount of recent development in the biological and physical procedures, development of statistical analysis techniques has failed to keep pace. The aim of this paper is to review the current state of the art in uncertainty analysis techniques across the 'EURADOS Working Group 10-Retrospective dosimetry' members, to give concrete examples of implementation of the techniques recommended in the international standards, and to further promote the use of Monte Carlo techniques to support characterisation of uncertainties. It is concluded that sufficient techniques are available and in use by most laboratories for acute, whole body exposures to highly penetrating radiation, but further work will be required to ensure that statistical analysis is always wholly sufficient for the more complex exposure scenarios.

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Section: Probabilistic Approach: Bayesian Methodsmentioning

confidence: 99%

Section: Biodosimetrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Uncertainty on Radiation Doses Estimated by Biological and Retrospective Physical Methods

Ainsbury

Samaga

Monaca

et al. 2017

Radiation Protection Dosimetry

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“…Efforts could be made to reduce uncertainties, but one might wonder if this is really necessary, regarding the contribution of the reference Table 2. Summary of reference doses used for the second inter-laboratory comparison (ILC) as in Barrios et al (2016) dosimetry to the total uncertainties budget in biological dosimetry. The target in terms of accuracy for such ILC programs remains to be discussed for biological applications and a reasonable target for accuracy has to be defined.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the influence of calibration practices on cytogenetic laboratory performance for dose estimation

Trompier

Baumann

Barrios

et al. 2016

International Journal of Radiation Biology

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Purpose: In the frame of the QA program of RENEB, an inter-laboratory comparison (ILC) of calibration sources used in biological dosimetry was achieved to investigate the influence of calibration practices and protocols on the results of the dose estimation performance as a first step to harmonization and standardization of dosimetry and irradiation practices in the European biological dosimetry network. Materials and methods: Delivered doses by irradiation facilities used by RENEB partners were determined with EPR/alanine dosimetry system. Dosimeters were irradiated in the same conditions as blood samples. A short survey was also performed to collect the information needed for the data analysis and evaluate the diversity of practices. Results: For most of partners the deviation of delivered dose from the targeted dose remains below 10%. Deviations larger than 10% were observed for five facilities out of 21. Origins of the largest discrepancies were identified. Correction actions were evaluated as satisfactory. The re-evaluation of some ILC results for the fluorescence in situ hybridization (FISH) and premature chromosome condensation (PCC) assays has been performed leading to an improvement of the overall performances. Conclusions: This work has shown the importance of dosimetry in radiobiology studies and the needs of harmonization, standardization in irradiation and dosimetry practices and educational training for biologists using ionizing radiation. ARTICLE HISTORY

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“…The first five assays rely on analysing peripheral blood lymphocytes and the last two assays on analysing components of personal electronic devices (PED), such as smartphones. The assays are described elsewhere Barquinero et al 2016;Depuydt et al 2016;Moquet et al 2016;Terzoudi et al 2016;Trompier et al 2016). An example of a dosimetric data set for 10 patients and the correct triage classification is shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

RENEB accident simulation exercise

Brzozowska

Ainsbury

Baert

et al. 2016

International Journal of Radiation Biology

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Purpose: The RENEB accident exercise was carried out in order to train the RENEB participants in coordinating and managing potentially large data sets that would be generated in case of a major radiological event. Materials and methods: Each participant was offered the possibility to activate the network by sending an alerting email about a simulated radiation emergency. The same participant had to collect, compile and report capacity, triage categorization and exposure scenario results obtained from all other participants. The exercise was performed over 27 weeks and involved the network consisting of 28 institutes: 21 RENEB members, four candidates and three non-RENEB partners. Results: The duration of a single exercise never exceeded 10 days, while the response from the assisting laboratories never came later than within half a day. During each week of the exercise, around 4500 samples were reported by all service laboratories (SL) to be examined and 54 scenarios were coherently estimated by all laboratories (the standard deviation from the mean of all SL answers for a given scenario category and a set of data was not larger than 3 patient codes). Conclusions: Each participant received training in both the role of a reference laboratory (activating the network) and of a service laboratory (responding to an activation request). The procedures in the case of radiological event were successfully established and tested. ARTICLE HISTORY

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RENEB biodosimetry intercomparison analyzing translocations by FISH

Abstract: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.

Cited by 24 publications

References 27 publications

Uncertainty on Radiation Doses Estimated by Biological and Retrospective Physical Methods

Uncertainty on Radiation Doses Estimated by Biological and Retrospective Physical Methods

Investigation of the influence of calibration practices on cytogenetic laboratory performance for dose estimation

RENEB accident simulation exercise

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