In this study, the deposit formation of B20-NA biodiesel with the addition of bioadditive Essential Oil with different variations, B20 + Essential Oil 1:2000, B20 + Essential Oil 1:3000, B20 + Essential Oil 1: 4000 and B20 + Essential Oil 1:5000 method of hot plate drops to know the characteristics and mechanisms of deposit formation on each variation of fuel. The research is carried out by the deposition process and evaporation of Diesel fuels carried out repeatedly on a hot plate. The plate is heated with temperature variations inside the enclosed space so that the conditions are close to the real conditions of the engine. This test uses a hot chamber test rig. The use of bioadditive essential oils (atsiri) which have turpentine contents bind water content and residues in biodiesel fuel is expected to accelerate the process of excessive deposit release in the combustion chamber. This study aims to find the optimum fuel and temperature variations so that the deposit release process can be accelerated.