Renin Gene Expression in Rat Tissues: A New Quantitative Assay Method for Rat Renin mRNA Using Synthetic cRNA

Suzuki, Fujio; Ludwig, Gerald M.; Hellmann, W.; Paul, Martin; Lindpaintner, Klaus; Murakami, Kazuo; Ganten, Detlev

doi:10.3109/10641968809103531

Cited by 46 publications

(20 citation statements)

References 24 publications

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“…As determined by quantitative PCR, renal reninmRNA expression was high with 1.74±0.2 pg renin-mRNA/ ,gg total renin RNA. This level is comparable to that reported in the literature with a range of 2.5-5.2 pg renin/,tg total RNA for rats and mice, respectively (20,21 ). In that PCR is extremely sensitive and allows quantification of specific mRNA expression from small tissue samples such as biopsy specimens, it may be of use to study changes in renal RAS gene expression in man under various in vivo conditions.…”

Section: Resultssupporting

confidence: 69%

“…The presence of renin mRNA in cardiac tissue, however, is still controversial. Whereas several investigators (20,21,24) have described specific mRNA expression by Northern blot and solution hybridization analysis in mouse and rat hearts, studies by Ekker et al (25) and Iwai and Inagami (18) were unable to detect cardiac renin mRNA. It is unclear at present ifthe apparent discrepancy is due to species differences, experimental conditions or differential regulation of renin.…”

Section: Resultsmentioning

confidence: 99%

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Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction.

Paul

Wagner²,

Dzau³

1993

J. Clin. Invest.

227

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Activation of tissue-specific gene expression of the components of the renin-angiotensin system (RAS) in humans may play an important role in cardiovascular regulation and pathophysiology. Studies of human tissue RAS expression, however, have been limited by the lack of availability of sufficient amounts of fresh human tissues and a sensitive method for detecting specific mRNAs. To demonstrate the presence of components of local RASs in humans we used the polymerase chain reaction (PCR) after reverse transcription to detect renin-, angiotensinogen-, and angiotensin-converting enzyme-mRNA in small quantities of human tissues. Results indicated that all components of the RAS were widely expressed in human organ samples. In order to study changes of gene expression in small tissue samples (e.g., renal biopsies) obtained from patients, we established a competitive PCR assay for quantification ofrenin, using a 155-basepair deletion mutant of the human renin cDNA as an internal standard. Renin-mRNA concentration was quantitated in the kidney (1.74±0.2 pg renin/,gg total RNA), adrenal gland (1.15±0.15 pg renin/gg total RNA), placenta (0.7±0.1 pg renin/,gg total RNA), and saphenous vein (0.02±0.01 pg renin/gg total RNA). The method described here may serve as a highly sensitive tool to quantify alterations in gene expression in man under various pathophysiologic conditions. This study should provide the methodological basis for future studies of tissue RAS in human physiology and disease. (J. Clin.

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction.

Paul

Wagner²,

Dzau³

1993

J. Clin. Invest.

227

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“…In another study, coadministration of dexamethasone, estradiol and triiodothyronine accumulated mRNA in several tissues including the mesentery (Campbell and Habener 1986 (Suzuki et al, 1988). These authors explained the dissociation of mRNA and its related product by differences in translational efficiency among various organs, or differences in post-translational processing.…”

Section: Discussionmentioning

confidence: 98%

Angiotensin II Generation in Mesenteric Arteries in Rats: Effects of Nephrectomy, Deoxycorticosterone and Dexamethasone.

et al. 1990

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“…1 " 6 The colocalization of Ang II in these tissues 7 -10 has led to the proposal of tissue reninangiotensin systems whose role might be to produce Ang II for local action (for review, see References 11 and 12). However, the precise mechanisms governing the local generation of Ang II in tissues have not been fully explored, and there is still some debate whether Ang II is synthesized intracellularly or extracellularly.…”

Section: Alternative Mechanisms Must Explain How Angiotensin II Is Symentioning

confidence: 99%

Rat angiotensinogen is secreted only constitutively when transfected into AtT-20 cells.

Deschepper

Reudelhuber

1990

Hypertension

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To test whether angiotensinogen might be targeted to dense core secretory granules in cells containing a regulated secretory pathway, we expressed rat angiotensinogen in AtT-20 cells, a mouse pituitary cell line that has the demonstrated ability to correctly sort proteins to the constitutive or regulated pathway. We compared the pattern of secretion of angiotensinogen with that of endogenous adrenocorticotropin hormone, which is secreted by AtT-20 cells through the regulated pathway. When cells were incubated for 5 hours with dibutyryladenosine cyclic monophosphate or KCI, adrenocorticotropin hormone secretion was significantly higher than control, whereas monensin had no effect In contrast, angiotensinogen secretion was markedly reduced by monensin, but no stimulation was observed with dibutyryladenosine cyclic monophosphate or KCI. These results make it unlikely that angiotensinogen could be cotargeted with active renin in the dense core granules of the regulated pathway. Alternative mechanisms must explain how angiotensin II is synthesized locally by tissue renin-angiotensin systems. (Hypertension 1990;16:147-153)

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Renin Gene Expression in Rat Tissues: A New Quantitative Assay Method for Rat Renin mRNA Using Synthetic cRNA

Cited by 46 publications

References 24 publications

Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction.

Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction.

Angiotensin II Generation in Mesenteric Arteries in Rats: Effects of Nephrectomy, Deoxycorticosterone and Dexamethasone.

Rat angiotensinogen is secreted only constitutively when transfected into AtT-20 cells.

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