Reparative Mineralized Tissue Characterization after Direct Pulp Capping with Calcium-Silicate-Based Cements

Abstract

Nowadays, the preservation of dental pulp vitality is an integral part of our daily therapies. The success of these treatments depends on the clinical situation as well as the biomaterials used. Mineral Trioxide aggregate and BiodentineTM are commonly used as pulp capping materials. One objective of vital pulp therapy is the repair/regeneration of the pulp. In addition to the initial inflammatory status of the pulp, the nature and quality of the new mineralized tissue obtained after pulp capping directly influence the success of the treatment. In order to characterize the reparative dentin, in the current study, the chemical composition and microstructure of the dentin bridge after direct pulp capping using Biodentine™ and mineral trioxide aggregate (MTA) was studied by using Raman microspectroscopy and scanning electron microscopy, respectively. The results showed that the reparative dentin bridge observed in both groups presented dentin tubules and chemical composition similar to primary dentin. With the limitations of this study, the calcium-silicate-based cements used as pulp capping materials provide an optimal environment for pulp healing, resulting in a reparative dentin resembling on certain points of the primary dentin and the regeneration of the pulp.

Shear Bond Strength of Nanohybrid Composite to Biodentine with Three Different Adhesives

“…Calcium hydroxide is not completely accepted as a pulp capping material due to its unpredictable results, towing to the poor quality of dentin bridges and the low sealing to dentine walls [11,14,15]. On the other hand, MTA has superior clinical performance for its ability to induce the formation of a regular dense mineralized tissue.…”

“…On the other hand, MTA has superior clinical performance for its ability to induce the formation of a regular dense mineralized tissue. The chemical composition of dentin bridges after pulp capping with MTA is similar to primary dentin [14]; however, it has some disadvantages such as a long setting time, poor handling properties, high material costs, and discoloration of dental tissue [16].…”

“…Zircona is added as a contrast medium [21]. When compared with MTA, it has better sealing, a high compression resistance, a low setting time, no discoloration, and major biocompatibility and bioactivity [11,14,[22][23][24]. Furthermore, Biodentine ® induces transforming growth factor-β1 (TGF-β1), facilitates odontoblast differentiation, and stimulates reparative dentine formation, under the form of expressed markers of odontoblasts and osteodentine [23].…”

The effects of mineral trioxide aggregate on osteo/odontogenic potential of mesenchymal stem cells: a comprehensive and systematic literature review

“…While cell and molecular signalings involved in the migration of dental pulp progenitor cells during natural reparative dentinogenesis are not fully understood, recent studies focused on strategies mimicking these natural phenomena [53][54][55][56][57]. Similar to well-known hematopoietic stem cell transplantation procedure, it has faced many difficulties in developing regenerative approaches.…”

Efeito da associação de meio condicionado por células tronco de polpa dentária humana e MTA ProRoot no reparo de polpas expostas de ratos

“…O Biodentine está associado a um alto pH (12) e à liberação de íons cálcio e silício, o que estimula a mineralização e cria uma "zona de infiltração mineral" ao longo da sua interface com a dentina, proporcionando uma melhor vedação. Caron et al 2014 (Awawdeh et al, 2018;Mahmoud et al, 2018;Tran et al, 2019).…”