Repeatability and reproducibility of post-mortem central corneal thickness measurements using a portable optical coherence tomography system in humans: a prospective multicenter study

Abstract

To assess the repeatability and reproducibility of post-mortem central corneal thickness (CCT) measurements made by the portable iVue spectra-domain (SD) optical coherence tomography OCT (Optovue Inc, Fremont, CA) system in humans, and to prospectively establish the time-course of CCT after death. In a prospective multicenter setting, CCT measurements were obtained from 58 human eyes at the following 16 time-points after death: immediately (within 2 h), and at each hour by the next 17 h. The range of CCT values for each subject was determined and longitudinal data were used to illustrate the variation in open and close eye mode. All measurements were made by two independent and well-trained examiners for session. Main outcome measures were intraclass correlation coefficients (ICC), repeatability and reproducibility coefficients, and coefficients of variation of the average central (0–2 mm). Overall, a total of 5,568 OCT measurements were performed by examiners. The repeatability coefficient varied from 0.3 to 1.7% and the reproducibility coefficient varied from 0.3 to 1.6% throughout the entire experimental time frame. Furthermore, the values of the different ICCs were also high during the different postmortem intervals, thus demonstrating the excellent repeatability and reproducibility of the present OCT approach. When CCT measurements were analyzed longitudinally, corneal thickness showed different behavior based on the open or close eye mode. The present study demonstrates that portable OCT imaging can be reliably used for corneal pachymetric measurements in supine subjects and during the post mortem period, i.e. without visual fixation and normal physiology/architecture of examined tissues.

High-Resolution Optical Coherence Tomography Angiography Characteristics of Limbal Stem Cell Deficiency

“…Hopefully with further advances in HR-OCT platforms there will be automated settings for the anterior segment angiography scans and integrated software for reflectivity and vascular density assessment, which will significantly reduce the post-processing time and improve the applicability of this modality. Previous studies have shown good repeatability and reproducibility for measuring parameters such as central corneal thickness using HR-OCT [ 26 ]. However, as we aimed to explore the OCT characteristics of LSCD in this study, we did not look at the intra and interobserver variability.…”

<p>Repeatability and Interchangeability of Topometric, Anterior Chamber and Corneal Wavefront Data Between Two Scheimpflug Camera Devices</p>

“…Its portability in the supine position, plus the ability to record high-resolution scans for monitoring changes, and the ability for rapid and non-contact acquisition, make portable OCT imaging a valuable tool. 25 Trefoil-90 (G) and Trefoil-0 (H). The X-axis represents the mean of the readings recorded by both devices (device 1 + device 2/2), while the Y-axis represents the difference between the mean device 1 and device 2 readings (device 1 -device 2).…”

Role of Immune Cell Diversity and Heterogeneity in Corneal Graft Survival: A Systematic Review and Meta-Analysis

“…Corneal transplantation has been proven to be the most effective therapy for corneal disorders such as opacity, keratoconus, corneal degeneration, scarring due to keratitis, trauma, and any physio-pathological changes on the ocular surface [1][2][3]. In addition to the appropriate examination of the characteristics of the donor and the tissue [4], immune privilege plays an important role in the success of corneal transplantation procedures [5,6].…”