Repeatability and Reproducibility of Quantitative Corneal Shape Analysis after Orthokeratology Treatment Using Image-Pro Plus Software

Abstract

Purpose. To evaluate the repeatability and reproducibility of quantitative analysis of the morphological corneal changes after orthokeratology treatment using “Image-Pro Plus 6.0” software (IPP). Methods. Three sets of measurements were obtained: two sets by examiner 1 with 5 days apart and one set by examiner 2 on the same day. Parameters of the eccentric distance, eccentric angle, area, and roundness of the corneal treatment zone were measured using IPP. The intraclass correlation coefficient (ICC) and repetitive coefficient (COR) were used to calculate the repeatability and reproducibility of these three sets of measurements. Results. ICC analysis suggested “excellent” reliability of more than 0.885 for all variables, and COR values were less than 10% for all variables within the same examiner. ICC analysis suggested “excellent” reliability for all variables of more than 0.90, and COR values were less than 10% for all variables between different examiners. All extreme values of the eccentric distance and area of the treatment zone pointed to the same material number in three sets of measurements. Conclusions. IPP could be used to acquire the exact data of the characteristic morphological corneal changes after orthokeratology treatment with good repeatability and reproducibility. This trial is registered with trial registration number: ChiCTR-IPR-14005505.

Influence of Overnight Orthokeratology on Corneal Surface Shape and Optical Quality

“…The SRI reflects the optical quality of the central cornea, and it is correlated with visual acuity, whereas the SAI reflects the asymmetry and local abnormal increases in corneal power [ 16 ]. The SRI is used to distinguish whether a reduced visual acuity results from a change in corneal topography or not, and the SAI is used as a quantitative parameter for monitoring changes in corneal topography [ 16 ].The results supported the previous reports that corneal topography is more irregular after remodeling the cornea [ 17 ] Kobayashi et al [ 16 ] studied patients fitted with the overnight reverse geometry orthokeratology lenses and followed them up for 52 weeks and found that overnight orthokeratology not only improved uncorrected visual acuity but also increased corneal irregularity.…”

A self‐organized actomyosin drives multiple intercellular junction disruption and directly promotes neutrophil recruitment in lipopolysaccharide‐induced acute lung injury

“…The positive signals were detected by a Diaminobenzidine Peroxidase Substrate Kit (Agilent Technologies, Santa Clara, CA, USA) and then counterstained with hematoxylin. The microphotographs of the sections were taken with a light microscope (Olympus) and quantified by Image-Pro plus 6.0 (Media Cybernetics, Rockville, MD, USA) followed by immunochemistry data analysis protocol (31). For phalloidin, MLC2, p-MLC2, ROCK1, p-ROCK1, MYLK, p-MLCK, MYPT1, p-MYPT1, profilin1, and Tb4 are expressed in neutrophils, which vary greatly during ALI, so their relative intensity was calculated by the total intensity in 1 arbitrary alveoli divided by the total area of cells in that region relative to the control group.…”

Effects of corneal crosslinking on corneal shape stabilization after orthokeratology

“…In subtractive maps, the reshape zone is located in the central circular zone and surrounded by the ring of mid-peripheral corneal steepening. According to a previous study 34 , it can be effectively determined using a custom step size setting of 0.1 D in Medmont Studio 6 software (Medmont Pty Ltd., Victoria, Australia). The shape of the reshape zone was portrayed and copied in each tangential map with Adobe Photoshop software.…”