Repetitive action potentials induced in Arabidopsis thaliana leaves by wounding and potassium chloride application

Favre, Patrick; Greppin, Hubert; Agosti, Robert Degli

doi:10.1016/s0981-9428(01)01317-1

Cited by 22 publications

(20 citation statements)

References 35 publications

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“…Since the APs sometimes run very fast, this biphasic characteristic cannot be recognized clearly in all of the shown kinetics. It is stressed that the experiments with KCl presented here are not to be compared with those carried out by Favre et al (2001) on Arabidopsis leaves, where repetitive APs were triggered through the addition of 250 mM or higher concentrations of KCl following pricking. Action potentials are triggered by diVerent factors All agents tested depolarize the cells at the site of application.…”

Section: Basic Responsesmentioning

confidence: 98%

Systemic signalling in barley through action potentials

Felle

Zimmermann

2007

Planta

153

122

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Using apoplastic voltage- and ion selective microprobes, in barley leaves action potentials (APs) have been measured, which propagate acropetally as well as basipetally from leaf to leaf or from root to leaf following the application of mild salt stress (e.g. 30-50 mM KCl or NH(4)Cl) or amino acids (e.g. 1 mM glutamic acid or 5 mM GABA). Voltage changes were biphasic, followed an 'all-or-none' characteristic, and propagated at 20-30 cm min(-1) irrespective of the direction. With the salt-induced APs, a strong initial depolarization is the main AP-releasing factor that first causes Ca(2+) influx and then anion efflux. Ca(2+) influx coincides with an initial slower depolarization, the rapid anion efflux causes the typical voltage 'break-through'. Subsequently, K(+)-efflux starts after the depolarizing voltage has passed the K(+) equilibrium potential (inversion of the K(+) driving force). Glutamic acid and GABA induce APs not through membrane depolarization, but presumably by binding to a putative receptor or to ligand-gated Ca(2+)-conducting channels, respectively, followed by Ca(2+) induced activation of anion efflux. APs are accompanied by transient apoplastic pH increase (about 1 unit), and by cytoplasmic pH decrease (about 0.5 units). The apoplastic pH change is interpreted as an indicator of stress, the cytoplasmic pH change as a prerequisite for defence related gene activation. Since APs are released by agents added in a moderate concentration range, it is suggested that they may serve as first and fast systemic signals following attack from pathogens.

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Section: Basic Responsesmentioning

confidence: 98%

Systemic signalling in barley through action potentials

Felle

Zimmermann

2007

Planta

153

122

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“…It was already shown that various mechanical injuries, such as pinching in Arabidopsis (Favre et al, 2001) and cutting in V. faba (Furch et al, 2008) and Cucurbita maxima , triggered elRs near the site of stimulus (s = 30-90 mm). However, we were not able to confirm the presence of elRs in distant target leaves using diverse types of leaf damage: cutting (razor blade or scissors), pricking (needle), picking (forceps), squeezing (tubes), or robotic punching with the so-called MecWorm.…”

Section: Insect Feeding a Two-component Processmentioning

confidence: 99%

Herbivore-Triggered Electrophysiological Reactions: Candidates for Systemic Signals in Higher Plants and the Challenge of Their Identification

et al. 2016

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In stressed plants, electrophysiological reactions (elRs) are presumed to contribute to long-distance intercellular communication between distant plant parts. Because of the focus on abiotic stress-induced elRs in recent decades, biotic stress-triggered elRs have been widely ignored. It is likely that the challenge to identify the particular elR types (action potential [AP], variation potential, and system potential [SP]) was responsible for this course of action. Thus, this survey focused on insect larva feeding (Spodoptera littoralis and Manduca sexta) that triggers distant APs, variation potentials, and SPs in monocotyledonous and dicotyledonous plant species (Hordeum vulgare, Vicia faba, and Nicotiana tabacum). APs were detected only after feeding on the stem/culm, whereas SPs were observed systemically following damage to both stem/culm and leaves. This was attributed to the unequal vascular innervation of the plant and a selective electrophysiological connectivity of the plant tissue. However, striking variations in voltage patterns were detected for each elR type. Further analyses (also in Brassica napus and Cucurbita maxima) employing complementary electrophysiological approaches in response to different stimuli revealed various reasons for these voltage pattern variations: an intrinsic plasticity of elRs, a plant-specific signature of elRs, a specific influence of the applied (a)biotic trigger, the impact of the technical approach, and/or the experimental setup. As a consequence, voltage pattern variations, which are not irregular but rather common, need to be included in electrophysiological signaling analysis. Due to their widespread occurrence, systemic propagation, and respective triggers, elRs should be considered as candidates for longdistance communication in higher plants.

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“…In this plant, AP presence has been reported after temperature treatment (Krol et al, 2004). For the C24 accession, we have shown that repetitive action potentials (RAPs) are elicited following leaf wounding and the subsequent application of 1 M KCl (Favre et al, 2001). Moreover, membrane potential excitability in intact Arabidopsis plants has been demonstrated by electrical stimulation in the same manner as classical animal nerve studies (Favre and Degli Agosti, 2007).…”

Section: Introductionmentioning

confidence: 74%

“…The original sampling rate was 10 Hz, and five acquired points were averaged to result in 2 Hz acquisition data. Detailed methods have been described in previous publications (Favre et al, 2001;Favre and Degli Agosti, 2007).…”

Section: Methods Of Measurementmentioning

confidence: 99%

“…These results suggest that specific genetic characteristics may play a significant role in determining Arabidopsis AP responses after abiotic stimulation (excitability). We tested this observation further by comparing a large number of plants (100 each) from the Col, Ws and Ler accessions after the same wounding and 1 M KCl treatment as described in Favre et al (2001) and report the results in the present paper.…”

Section: Introductionmentioning

confidence: 99%

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