Repetitive sequences in the rat genome that have cleavage sites for the restriction endonuclease Taq I.

Abstract

A cleavage of rat DNA with restriction endonuclease Tag I produced at least 8 discrete bands. One of the bands, 0.29 kilobase pairs (kb) long, was isolated and cloned in plasmid. The hybridization of this fragment to genomic DNA digested with Tag I showed 1.6 kb, 1.2 kb and 0.29 kb band in a smear hybridization signal. A repetition of the DNA sequence of this fragment was about 12,500 copies haploid genome of both Buffalo and Sprague-Dawley rats by the Cot-hybridization analysis. Four independent clones of 0.29 kb Tag I fragment share high homology (97%). repetitive sequence ; restriction endonuclease Tag I ; rat Recent developments in the field of molecular oncology have facilitated the detection of potent oncogenes by the method of DNA transfection to mouse 3T3 cells (Cooper 1982). In these experiments, repetitive DNA of the donor animal, such as the Alu-sequence in the human genome (Jelinek and Schmid 1982), is usually used to detect the integrated donor DNA in the transformants. In the case of the rat genome, there was no suitable sequence for this purpose.