Replacing antibodies with aptamers in lateral flow immunoassay

A specific aptamer-cell penetrating peptides complex delivered siRNA efficiently and suppressed prostate tumor growthin vivo

“…29 Aptamers have the advantages of being highly specific, relatively small in size, and easily synthesized, which could be regarded as a chemical equivalent of antibodies. 30,31 In this study, we adopted a truncated aptamer A10-3.2 with 39 nucleotides for its simplicity of chemical synthesis and enhanced specificity. It is about 55% of the full A10 in size.…”

Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

“…Aptamers have been used as 'antibody mimics' or alternatives to antibodies in some chromatographic binding assays [17][18][19][20]. These binding agents consist of single-stranded DNA or RNA sequences that are often 10 to 100 nucleotides in length and that have the ability to form highly ordered 3D structures [19].…”

“…These binding agents consist of single-stranded DNA or RNA sequences that are often 10 to 100 nucleotides in length and that have the ability to form highly ordered 3D structures [19]. Aptamers are prepared through a selection and amplification process known as SELEX (i.e., the 'systematic evolution of ligands by exponential enrichment'), which can be used to generate specific binding agents with relatively high affinities for a particular target [17,18].…”

“…Aptamers are prepared through a selection and amplification process known as SELEX (i.e., the 'systematic evolution of ligands by exponential enrichment'), which can be used to generate specific binding agents with relatively high affinities for a particular target [17,18]. These agents have been used to bind targets that have included drugs, peptides, proteins and cells [17][18][19][20]. It is possible to easily modify the structure of an aptamer to include a label or to immobilize the aptamer to a support [18,19].…”

“…These agents have been used to bind targets that have included drugs, peptides, proteins and cells [17][18][19][20]. It is possible to easily modify the structure of an aptamer to include a label or to immobilize the aptamer to a support [18,19]. In addition to being used in biosensors [17,18], aptamers have been employed in chromatography for both the purification of proteins [20] and in various forms of chromatographic binding assays [19].…”