Replica Plating and Rapid Ascus Collection of Neurospora

Maling, Barbara D.

doi:10.1099/00221287-23-2-257

Cited by 17 publications

(9 citation statements)

References 4 publications

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“…A method using a board with closely arranged needles instead of velvet was invented by FORBES (as described by ROBERTS 1959) for replication of Aspergillus. It has also in some instances been of use for replication of Neurospora (MALING 1960).…”

Section: Replication By the Net Methodsmentioning

confidence: 99%

Glycerol non-utilizing mutants in Neurospora crassa

Nilheden¹,

Holm²,

Kolmark³

2009

Hereditas

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The net replication method. Methods were developed for screening of glycerol non-utilizing mutants from Neurospora crassa. The strains utilized produce only mononucleate conidia (pefi, microconidia). Some strains were inherently colonial, independent of temperature, others were of the cot (colonial, temperature-sensitive) type. Mutagen (ultraviolet light) treated conidia were spread on plates with medium containing glucose as the source of carbon, and overlaid with a disc of Terylene net. Outgrowing colonies were transferred with the nets to plates with glycerol as carbon. Replication was accomplished by a period of growth. Non-replicating colonies were isolated from the glucose plates and retested as presumptive glycerol non-utilizing mutants.GfyceroI non-utilizing mutants. One glycerol non-utilizing mutant, gfy-u (234), appears otherwise physiologically normal and segregates as a nuclear gene in crosses. Another, gly-u (245), has a very long lag period (ca 2-3 weeks) on glycerol medium, but eventually adapts itself to growth on this carbon source. Photographs of replicated colonies as well as mutants are presented. The biochemical pathways of glycerol metabolism are outlined from work by various authors, and the present studies are discussed in this connection.

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Section: Replication By the Net Methodsmentioning

confidence: 99%

Glycerol non-utilizing mutants in Neurospora crassa

Nilheden¹,

Holm²,

Kolmark³

2009

Hereditas

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“…Produces very short conidiophores, bearing conidia in tight clusters (610,611). Photographs (533,634). Recessive.…”

Section: Con-i: Recombination Control-1mentioning

confidence: 99%

“…Modifier mutations which alter morphology and the ability of cr-i to use glycerol occur frequently (383,905). Crosses homozygous for allele B123 exude intact linear asci (634). Double mutants sn cr and cr rg form small conidiating colonies suitable for replica plating with velvet (182,634,796,932,1020).…”

Section: Con-i: Recombination Control-1mentioning

confidence: 99%

Chromosomal loci of Neurospora crassa

Perkins¹,

Radford²,

Newmeyer³

et al. 1982

Microbiol Rev

263

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I I ' ~~~~T h Ñ CiOr(r r N z ADDITIONAL LOCI IN LINKAGE GROUP ILinked to mt (8 to 12%) Linked to mt, acr-3 (5%) Right of arg-i (5/29) Linked to so and aro-8 Right of un-18 Between ad-5 and the centromere Between arg-i (4%) and his-3 (I to 2%) Between In(H4250) and T(39311) Left of mt (23%) Linked to mt (2%) and aza-i (39%o) Left of nit-2 (30%) Left of mt (14%) Linked to un-i (9%), probably to the right Linked to hom (1%), probably to the right Linked to mt (17 to 22%) Between T(NM103) and al-2 (18%) Between T(4540) and cr-2Between thi-i (12 to 20%/6) and ad-9 (5%) Linked to ad-5 (1/54), probably to the left Linked to mt (6%) Linked to cys-5 (<1%), probably to the right Right of ad-9 (12%), linked to al (0/76) Right of his-3 (2%) Between the T(AR173) breakpoints Linked to al-2 (10%) and nic-i (1 to 5%) Linked to ad-9 (0/44); right of thi-i (2%) Linked to mt (5%), probably to the left Linked to al-I (10%) Right of os-i (3%) Between nit,i (5 to 19%) and al-I (6 to 19%io) Linked near arg-i Left of al-2 (25%o) Left of mt (16%) Right of nic-2 (6%) Linked to ad-9 (2%), probably to the right Left of cr-i (15%) Left of mt (10%) Probably between his-2 (3%) and cr-i (3%) Between T(NM103) and the right tip Between mt (20%) and arg-i (1%) Between ad-3 (6%) and al-i (16%) Between his-3 and nic-2 Between cyh-i (5%) and al-I (7%) Linked to cyh-i (18%), al-i (2%), and mb-2 (6%) Between arg-i (3%) and T(39311) Between tre (41%) and ad-9 Linked to mt (9%) Linked to mt (20%) Complex phenotype. Alternative requirements: 2-acetylaminofluorine, certain azo dyes, or certain single amino acids. Cold sensitive. Originated among progeny of a rib-i strain that had become tolerant to 2-acetylaminofluorine.

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“…Mackintosh & Pritchard (1963) found that with the induction of micro-colonies with sodium deoxycholate and the use of damp velveteen they were able to replica plate Aspergillus nidulans. Other methods have also proved effective -the use of a perspex block embedded with steel pins (Roberts, 1959) or filter paper (Maling, 1960), along with morphological mutants of Neurospora crassa, which produced restricted growth. Other methods have also proved effective -the use of a perspex block embedded with steel pins (Roberts, 1959) or filter paper (Maling, 1960), along with morphological mutants of Neurospora crassa, which produced restricted growth.…”

Section: Introductionmentioning

confidence: 99%

A technique for replica platingCoprinus lagopus, a filamentous fungus

Kaplan

Walls

1971

Genet. Res.

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The problem of replica plating filamentous fungi such as Coprinus lagopus is overcome by inducing micro-colonies with sodium deoxycholate and using ' Velcro', a hooked material, to replace velveteen in the standard replica plating technique. ' Velcro' is advantageous in that it has a regular pattern of closely spaced hooks which transfer small inocula from the colonies on the master plate.* Present address: School of Biological Sciences, University of East Anglia, Norwich.

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Replica Plating and Rapid Ascus Collection of Neurospora

Cited by 17 publications

References 4 publications

Glycerol non-utilizing mutants in Neurospora crassa

Glycerol non-utilizing mutants in Neurospora crassa

Chromosomal loci of Neurospora crassa

A technique for replica platingCoprinus lagopus, a filamentous fungus

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