“…S. pombe homothallic strain GP1596 (h 90 rec11-111 ade6-M26 ura4-294 leu1-32) was transformed to Leu þ with plasmid pYL84, containing the ade6-469 gene which can recombine with the chromosome to produce Ade þ recombinants. The resulting ᮊ 1997 Blackwell Science Ltd, Molecular Microbiology, 23, 869-878 The S. pombe stb element stabilizes the plasmid in mitotic and meiotic cells, and the S. pombe ars element aids mitotic propagation (Heyer et al, 1986). The plasmid contains the S. pombe ura4 þ gene for selection in S. pombe (Grimm et al, 1988), and the pUC19 amp and ori elements for selection and propagation in E. coli (Yanisch-Perron et al, 1985).…”