Replicative senescence, telomere shortening and cell proliferation rate in Gaddi goat's skin fibroblast cell line

Gupta, Neelam; Taneja, Ritu; Pandey, Alok; Mukesh, Manishi; Singh, Hari; Gupta, S.C.

doi:10.1016/j.cellbi.2007.05.004

Cited by 14 publications

(9 citation statements)

References 38 publications

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“…The fetal and AF-derived cells in buffaloes have already been found to express key pluripotency molecular markers Dev et al 2011). In this study in long term cultures cells were able to proliferate up to 75 days and their proliferation rate was more up to 10 passages then decreased later may be due to accumulations of more non-dividing cells, was confirmed by earlier study conducted by Gupta et al (2007) in continuous culture of goat fibroblasts cells.…”

Section: Discussionsupporting

confidence: 77%

Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

et al. 2012

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Stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. We report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, OCT-4, NANOG and SOX-2) from the amnion of water buffalo (Bubalus bubalis). The cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. The cytochemical staining revealed that pluripotent cells were capable of undergoing directed differentiation in vitro into osteocytes. It could be inferred that amnionderived pluripotent stem cell-like cells can be isolated, cultured for many passages and differentiated into mesoderm lineage, and may be an alternative source to mesenchymal stem cells. These cells can have applications in assisted reproduction, developmental biological and regenerative medicine.

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Section: Discussionsupporting

confidence: 77%

Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

et al. 2012

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“…It has been reported that due to inherent limitations in the mechanics of DNA replication, telomeres shorten at each cell division, and in the absence of telomerase, when telomere shortening reaches a critical limit, cells are susceptible to chromosomal aberrations such as end-to-end fusion and aneuploidy. In such a situation, the cells cease to divide and reach replicative senescence [40]. Treatment with TRF, however, protected against telomere shortening in senescent HDFs with concomitant increased in telomerase activity.…”

Section: Discussionmentioning

confidence: 99%

Tocotrienol‐Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts

Makpol

Durani

Chua

et al. 2011

BioMed Research International

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This study determined the molecular mechanisms of tocotrienol-rich fraction (TRF) in preventing cellular senescence of human diploid fibroblasts (HDFs). Primary culture of HDFs at various passages were incubated with 0.5 mg/mL TRF for 24 h. Telomere shortening with decreased telomerase activity was observed in senescent HDFs while the levels of damaged DNA and number of cells in G0/G1 phase were increased and S phase cells were decreased. Incubation with TRF reversed the morphology of senescent HDFs to resemble that of young cells with decreased activity of SA-β-gal, damaged DNA, and cells in G0/G1 phase while cells in the S phase were increased. Elongated telomere length and restoration of telomerase activity were observed in TRF-treated senescent HDFs. These findings confirmed the ability of tocotrienol-rich fraction in preventing HDFs cellular ageing by restoring telomere length and telomerase activity, reducing damaged DNA, and reversing cell cycle arrest associated with senescence.

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“…In the absence of telomerase, when telomere shortening reaches a critical limit, cells are susceptible to chromosomal aberrations such as end-to-end fusion and aneuploidy. In such a situation, the cells cease to divide and reach replicative senescence 5,6. Telomere length may restrict the replicative potential of hemopoietic cells contributing to the decline in immune function with age 7.…”

Section: Introductionmentioning

confidence: 99%

γ‐Tocotrienol Prevents Oxidative Stress‐Induced Telomere Shortening in Human Fibroblasts Derived from Different Aged Individuals

Makpol

Abidin

Sairin

et al. 2009

Oxidative Medicine and Cellular Longevity

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The effects of palm γ-tocotrienol (GGT) on oxidative stress-induced cellular ageing was investigated in normal human skin fibroblast cell lines derived from different age groups; young (21-year-old, YF), middle (40-year-old, MF) and old (68-year-old, OF). Fibroblast cells were treated with γ-tocotrienol for 24 hours before or after incubation with IC50 dose of H2O2 for 2 hours. Changes in cell viability, telomere length and telomerase activity were assessed using the MTS assay (Promega, USA), Southern blot analysis and telomere repeat amplification protocol respectively. Results showed that treatment with different concentrations of γ-tocotrienol increased fibroblasts viability with optimum dose of 80 µM for YF and 40 µM for both MF and OF. At higher concentrations, γ-tocotrienol treatment caused marked decrease in cell viability with IC50 value of 200 µM (YF), 300 µM (MF) and 100 µM (OF). Exposure to H2O2 decreased cell viability in dose dependent manner, shortened telomere length and reduced telomerase activity in all age groups. The IC50 of H2O2 was found to be; YF (700 µM), MF (400 µM) and OF (100 µM). Results showed that viability increased significantly (p < 0.05) when cells were treated with 80 µM and 40 µM γ-tocotrienol prior or after H2O2-induced oxidative stress in all age groups. In YF and OF, pretreatment with γ-tocotrienol prevented shortening of telomere length and reduction in telomerase activity. In MF, telomerase activity increased while no changes in telomere length was observed. However, post-treatment of γ-tocotrienol did not exert any significant effects on telomere length and telomerase activity. Thus, these data suggest that γ-tocotrienol protects against oxidative stress-induced cellular ageing by modulating the telomere length possibly via telomerase.

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Replicative senescence, telomere shortening and cell proliferation rate in Gaddi goat's skin fibroblast cell line

Cited by 14 publications

References 38 publications

Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

Tocotrienol‐Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts

γ‐Tocotrienol Prevents Oxidative Stress‐Induced Telomere Shortening in Human Fibroblasts Derived from Different Aged Individuals

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