Reply to Bareille et al. Are Viscoelastometric Assays of Old Generation Ready for Disposal? Comment on “Volod et al. Viscoelastic Hemostatic Assays: A Primer on Legacy and New Generation Devices. J. Clin. Med. 2022, 11, 860”

Volod, Oksana; Bunch, Connor M.; Miller, Joseph; Moore, Ernest E.; Moore, Hunter B.; Kwaan, Hau C.; Patel, Shivani; Wiarda, Grant; Aboukhaled, Michael; Thomas, Scott; Fulkerson, Daniel H.; Erdman, Lee; Tincher, Anna; Walsh, Mark

doi:10.3390/jcm12020478

Cited by 2 publications

(1 citation statement)

References 67 publications

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“…The technique overcomes the limitations of both conventional PT and VHA techniques such as TEG or ROTEM 11 , 12 . A criticism of clinically-available VHA methodologies is that they considerably deform the clot sample, as they use either a rotating cup and pin system (e.g., TEG 5000, ROTEM delta, and ROTEM sigma), vertical cup and pin system (e.g., Sonoclot), or vibration detection by light emitting diode (e.g., TEG 6s) 21 – 23 . New and emerging technologies of point-of-care (POC) hemostatic assays have used iterations of ultrasonic deformation methodology 12 .…”

Section: Discussionmentioning

confidence: 99%

Multichannel Resonant Acoustic Rheometry System for Rapid and Efficient Quantification of Human Plasma Coagulation

Hendren,

Li,

Stegemann

et al. 2023

Preprint

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Resonant Acoustic Rheometry (RAR), a newly developed ultrasound-based technique for non-contact characterization of soft viscoelastic materials, has shown promise for quantitative assessment of plasma coagulation by monitoring the entire dynamic process in real time. Here, we report the development of a multichannel RAR (mRAR) system for simultaneous monitoring of the coagulation of multiple small-volume plasma samples, a capability that is critical to efficiently provide improved assessment of coagulation. The mRAR system was constructed using an array of 4 custom-designed ultrasound transducers at 5.0 MHz and an electronic driving system that controlled the generation of synchronized ultrasound pulses for real time monitoring of multiple samples simultaneously. The mRAR system was tested using Coumadin-treated plasma samples with a range of International Normalized Ratio (INR) values, as well as normal pooled plasma samples. Tracking of dynamic changes in clotting of plasma samples triggered by either kaolin or tissue factor was performed for the entire duration of coagulation. The mRAR system captured distinct changes in the samples and identified parameters including clotting time, clotting speed, and the mechanical properties of the clots that were consistent with Coumadin dose and INR levels Data from this study demonstrate the feasibility of the mRAR system for the rapid, efficient, and accurate characterization of plasma coagulation.

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Section: Discussionmentioning

confidence: 99%

Multichannel Resonant Acoustic Rheometry System for Rapid and Efficient Quantification of Human Plasma Coagulation

Hendren,

Li,

Stegemann

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Multichannel resonant acoustic rheometry system for quantification of coagulation of multiple human plasma samples

Hendren,

Li,

Stegemann

et al. 2023

Sci Rep

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Resonant Acoustic Rheometry (RAR), a newly developed ultrasound-based technique for non-contact characterization of soft viscoelastic materials, has shown promise for quantitative viscoelastic assessment of temporally changing soft biomaterials in real time, and may be used to monitor blood coagulation process. Here, we report the development of a novel, multichannel RAR (mRAR) system for simultaneous measurements of multiple temporally evolving samples and demonstration of its use for monitoring the coagulation of multiple small-volume plasma samples. The mRAR system was constructed using an array of 4 custom-designed ultrasound transducers at 5.0 MHz and a novel electronic driving system that controlled the generation of synchronized ultrasound pulses for real time assessment of multiple samples simultaneously. As a proof-of-concept of the operation of the mRAR system, we performed tests using pooled normal human plasma samples and anti-coagulated plasma samples from patients treated with warfarin with a range of International Normalized Ratio (INR) values as well-characterized samples with different coagulation kinetics. Our results show that simultaneous tracking of dynamic changes in 4 plasma samples triggered by either kaolin or tissue factor was achieved for the entire duration of coagulation. The mRAR system captured distinct changes in the samples and identified parameters including the clotting start time and parameters associated with the stiffness of the final clots that were consistent with INR levels. Data from this study demonstrate the feasibility of the mRAR system for efficient characterization of the kinetic coagulation processes of multiple plasma samples.

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Reply to Bareille et al. Are Viscoelastometric Assays of Old Generation Ready for Disposal? Comment on “Volod et al. Viscoelastic Hemostatic Assays: A Primer on Legacy and New Generation Devices. J. Clin. Med. 2022, 11, 860”

Abstract: We are pleased to see that Bareille et al. have written a Commentary: “Are viscoelastometric assays of old generation ready for disposal?” [...]

Cited by 2 publications

References 67 publications

Multichannel Resonant Acoustic Rheometry System for Rapid and Efficient Quantification of Human Plasma Coagulation

Multichannel Resonant Acoustic Rheometry System for Rapid and Efficient Quantification of Human Plasma Coagulation

Multichannel resonant acoustic rheometry system for quantification of coagulation of multiple human plasma samples

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