Report of the Fourth International Havemeyer Workshop on Equid Herpesviruses (EHV) EHV‐1, EHV‐2 and EHV‐5

Kydd, Julia H.; Lunn, D. P.; Osterrieder, Nikolaus

doi:10.1111/evj.13141

Cited by 8 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even if EHV-2 pathogenicity is considered weaker, this virus also remains an infectious agent for the equine species. 28 Considering this and the high prevalence of GHV in our population, we recommend future systematic PCR screening of UC-MSCs for EHV-2 and EHV-5 without a need of PMB screening for those pathogens, even if UC-MSCs appear free of equine GHV contamination for the moment.…”

Section: Discussionmentioning

confidence: 93%

Biosafety Evaluation of Equine Umbilical Cord-Derived Mesenchymal Stromal Cells by Systematic Pathogen Screening in Peripheral Maternal Blood and Paired UC-MSCs

Denys

Léon

Robert³

et al. 2020

Biopreservation and Biobanking

View full text Add to dashboard Cite

Background: The growing interest in mesenchymal stromal cells (MSCs) in equine medicine, together with the development of MSC biobanking for allogeneic use, raises concerns about biosafety of such products. MSCs derived from umbilical cord (UC) carry an inherent risk of contamination by environmental conditions and vertical transmission of pathogens from broodmares. There is yet no report in the scientific literature about horses being contaminated by infected MSC products, and no consensus about systematic infectious screening of umbilical cord-derived mesenchymal stromal cells (UC-MSCs) to ensure microbiological safety of therapeutic products. Objectives: To develop a standard protocol to ensure UC-MSC microbiological safety and to assess the risk of vertical transmission of common intracellular pathogens from broodmares to paired UC-MSCs. Study Design and Methods: Eighty-four UC and paired peripheral maternal blood (PMB) samples were collected between 2014 and 2016. Sterility was monitored by microbiological control tests. Maternal contamination was tested by systematical PMB PCR screening for 14 pathogens and a Coggins test. In case of a PCR-positive result regarding one or several pathogen(s) in PMB, a PCR analysis for the detected pathogen(s) was then conducted on the associated UC-MSCs. Results: Ten out of 84 UC samples were contaminated upon extraction and 6/84 remained positive in primo culture. The remaining 78/84 paired PMB & UC-MSC samples were evaluated for vertical transmission; 37/78 PMB samples were PCR positive for Equid herpesvirus (EHV)-1, EHV-2, EHV-5, Theileria equi, Babesia caballi, and/or Mycoplasma spp. Hepacivirus was detected in 2/27 cases and Theiler Diseases Associated Virus in 0/27 cases (not performed on all samples due to late addition). All paired UC-MSC samples tested for the specific pathogen(s) detected in PMB were negative (37/37). Main Limitations: More data are needed regarding MSC susceptibility to most pathogens detected in PMB. Conclusions: In-process microbiological controls combined with PMB PCR screening provide a comprehensive assessment of UC-MSC exposure to infectious risk, vertical transmission risk appearing inherently low.

show abstract

Section: Discussionmentioning

confidence: 93%

Biosafety Evaluation of Equine Umbilical Cord-Derived Mesenchymal Stromal Cells by Systematic Pathogen Screening in Peripheral Maternal Blood and Paired UC-MSCs

Denys

Léon

Robert³

et al. 2020

Biopreservation and Biobanking

View full text Add to dashboard Cite

show abstract

“…EHV-1 causes frequent outbreaks of abortion and myeloencephalopathy worldwide, even in vaccinated horses (Figure 4). EHV-1 outbreaks have been reported for centuries and many cases are reported across Europe, in France, Great Britain and Belgium, in the United States, in New-Zealand, Australia, Chile, Argentina, Israel and United Arab Emirates [31][32][33]. EHM incidence has increased in most parts of the world, in Europe and North America, as well as in Oceania, Africa, and Asia [10,11,28].…”

Section: Equid Herpesvirusesmentioning

confidence: 99%

Viral Equine Encephalitis, a Growing Threat to the Horse Population in Europe?

Lecollinet

Pronost

Coulpier

et al. 2019

Viruses

View full text Add to dashboard Cite

Neurological disorders represent an important sanitary and economic threat for the equine industry worldwide. Among nervous diseases, viral encephalitis is of growing concern, due to the emergence of arboviruses and to the high contagiosity of herpesvirus-infected horses. The nature, severity and duration of the clinical signs could be different depending on the etiological agent and its virulence. However, definite diagnosis generally requires the implementation of combinations of direct and/or indirect screening assays in specialized laboratories. The equine practitioner, involved in a mission of prevention and surveillance, plays an important role in the clinical diagnosis of viral encephalitis. The general management of the horse is essentially supportive, focused on controlling pain and inflammation within the central nervous system, preventing injuries and providing supportive care. Despite its high medical relevance and economic impact in the equine industry, vaccines are not always available and there is no specific antiviral therapy. In this review, the major virological, clinical and epidemiological features of the main neuropathogenic viruses inducing encephalitis in equids in Europe, including rabies virus (Rhabdoviridae), Equid herpesviruses (Herpesviridae), Borna disease virus (Bornaviridae) and West Nile virus (Flaviviridae), as well as exotic viruses, will be presented.

show abstract

“…[2][3][4][5] The ACVIM commissioned an EHV-1 consensus statement in 2009, 6 and there have been a series of international workshops focused on the virus, the most recent of which was in 2018. 7 Although scientific investigation of the pathogenesis of diseases like EHM and their prevention continues, so do frequent outbreaks of disease in populations of horses across the world, including major outbreaks that have devastating effects on equine health and the equine industry. 8,9 The ongoing global effects of EHM led to the ACVIM Board of Regents charging the authors to revise the EHV-1 consensus statement.…”

Section: Introductionmentioning

confidence: 99%

“…Primary infection, or reactivation of latent infection during periods of stress, causes clinical disease including rhinopneumonitis, epidemic abortion in late gestation, neonatal foal death, and equine herpesvirus myeloencephalopathy (EHM) 2‐5 . The ACVIM commissioned an EHV‐1 consensus statement in 2009, 6 and there have been a series of international workshops focused on the virus, the most recent of which was in 2018 7 . Although scientific investigation of the pathogenesis of diseases like EHM and their prevention continues, so do frequent outbreaks of disease in populations of horses across the world, including major outbreaks that have devastating effects on equine health and the equine industry 8,9 …”

Section: Introductionmentioning

confidence: 99%

Updated ACVIM consensus statement on equine herpesvirus‐1

Lunn,

Burgess,

Dorman

et al. 2024

Veterinary Internal Medicne

Self Cite

View full text Add to dashboard Cite

Equine herpesvirus‐1 (EHV‐1) is a highly prevalent and frequently pathogenic infection of equids. The most serious clinical consequences of infection are abortion and equine herpesvirus myeloencephalopathy (EHM). The previous consensus statement was published in 2009 and considered pathogenesis, strain variation, epidemiology, diagnostic testing, vaccination, outbreak prevention and control, and treatment. A recent survey of American College of Veterinary Internal Medicine large animal diplomates identified the need for a revision to this original consensus statement. This updated consensus statement is underpinned by 4 systematic reviews that addressed key questions concerning vaccination, pharmaceutical treatment, pathogenesis, and diagnostic testing. Evidence for successful vaccination against, or effective treatment of EHV‐1 infection was limited, and improvements in experimental design and reporting of results are needed in future studies of this important disease. This consensus statement also updates the topics considered previously in 2009.

show abstract

Report of the Fourth International Havemeyer Workshop on Equid Herpesviruses (EHV) EHV‐1, EHV‐2 and EHV‐5

Cited by 8 publications

References 33 publications

Biosafety Evaluation of Equine Umbilical Cord-Derived Mesenchymal Stromal Cells by Systematic Pathogen Screening in Peripheral Maternal Blood and Paired UC-MSCs

Biosafety Evaluation of Equine Umbilical Cord-Derived Mesenchymal Stromal Cells by Systematic Pathogen Screening in Peripheral Maternal Blood and Paired UC-MSCs

Viral Equine Encephalitis, a Growing Threat to the Horse Population in Europe?

Updated ACVIM consensus statement on equine herpesvirus‐1

Contact Info

Product

Resources

About