Repression of the λ pcin promoter by integrative host factor

G, Griffo; Oppenheim, Amos B.; Gottesman, Max E.

doi:10.1016/0022-2836(89)90169-1

Cited by 44 publications

(18 citation statements)

References 29 publications

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“…IHF-binding sites have been identified in several bacterial (r 7~ promoter regions. These include the E. coli ilvG-MEDA operon, some bacteriophage Mu promoters, and the ~ PL and pcin promoters (Pereira et al 1988;van Rijn et al 1988;Peacock et al 1984;Griffo et al 1989). In the case of the ilvGMEDA operon, IHF binding exerts a repressive effect on the transcription of the ilvGpl pro-…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Integration host factor is required for the activation of developmentally regulated genes in Caulobacter.

Gober¹,

Shapiro²

1990

Genes Dev.

111

105

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Several temporally controlled flagellar genes in Caulobacter crescentus require a cr s4 promoter and upstream sites for transcription activation. We demonstrate here that in some of these genes, an AT-rich region containing an integration host factor (IHF) consensus binding site lies between the activator and the promoter, and that this region binds IHF in vitro. Analysis of mutations in the IHF-binding region of the hook operon demonstrated that an intact IHF-binding site is necessary for transcription in vivo. An adjacent and divergent promoter also has an IHF consensus sequence that binds IHF. The IHF and enhancer sites are 3' to the transcription start site in this promoter. We postulate that IHF mediates the formation of a higher order structure between the divergent promoter regions in a manner analogous to the nucleosome-like structure generated for k-Escherichia coli DNA recombination and that this higher order structure modulates transcription.

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Section: Discussionmentioning

confidence: 99%

“…Transcription of this promoter only occurs in mutants lacking IHF or in vitro in the absence of IHF (Periera et al 1988). The X Pcin promoter may be repressed by IHF binding in a similar fashion (Griffo et al 1989). In contrast, IHF binding to the Mu and X PL promoters stimulates transcription.…”

Section: Genes and Development 1499mentioning

confidence: 99%

Integration host factor is required for the activation of developmentally regulated genes in Caulobacter.

Gober¹,

Shapiro²

1990

Genes Dev.

111

105

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“…The binding of IHF to specific DNA sequences at the K attachment site promotes A integration and excision. In a role that is probably related to DNA bending, IHF bound at K promoters can inhibit (22) or stimulate (18) gene expression.…”

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confidence: 99%

HU and integration host factor function as auxiliary proteins in cleavage of phage lambda cohesive ends by terminase

1991

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HU and iategrtion host factor (IHF) are small, basic heterodimeric DNA-binding proteins which participate in transcripion Intatin, DNA replication, and recom . We constructed isogenic Escherichia coli stains in which fIU, THF, or both proteins were absent. Bacteriophage X did not grow in hosts lacing both HU and THE. Phage DNA replication and late gene transcription were normal in the double mutants, but packaging of A DNA was defective. Mature phage DNA molecules were absent, indicating that terminase was unable to linearize K DNA. Phage variants carrying a small substitution near cos or the ohml mutation in the terminase gene, Nul, formed plaques on HU-IHF-strains. We propose that HU or THU is required to establish the higher-order DNA-protein structure at cos that is the substrate for K terminase.DNA-protein complexes with a high degree of organization form at the initiation of DNA replication and are intermediates in several DNA recombination reactions. The ordered structures arise from the concerted assembly of a number of proteins on supercoiled DNA and bring nucleotide sequences located at a distance or on separate molecules into close proximity. In some of these reactions, the DNA bending required to form the complexes is facilitated by low-molecular-weight basic DNA-binding proteins (reviewed by Travers [50]).The best-studied Escherichia coli proteins of this type are the integration host factor (IHF) and HU (reviewed by Drlica and Rouviere-Yaniv [7] and Friedman [14]). IHF is composed of two nonidentical subunits, a and ,B, encoded by the himA (32) and hip (12) genes, respectively. IHF binds in the DNA minor groove (54) and imparts a bend to the DNA in excess of 1400 (27,36,38,49). The binding of IHF to specific DNA sequences at the K attachment site promotes A integration and excision. In a role that is probably related to DNA bending, IHF bound at K promoters can inhibit (22) (10). Although k packaging can take place in THF-hosts, an absolute requirement for IHF can be seen when the phage carries a cos site mutation (cosl54 or cos59) (1,31) or when the host canries a DNA gyrase mutation (15). Phage carrying the ohml mutation in the Nul gene will form plaques on IHF-gyrB hosts (21; also see reference 9).The genes coding for the a and ,B subunits of HU, hupA and hupB, respectively, have recently been cloned (25,26

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“…Since the Ϫ53 mutation lies within one putative IHF binding site, we tested whether IHF is involved in the acetoacetate-induced expression of atoDAEB. Plasmids carrying the lacZ reporter under the control of either the wild-type promoter (atoD1) or a promoter with one mutation (Ϫ53 or Ϫ140) were introduced into both the wild-type E. coli strain N99 and its isogenic counterpart, N7193 (Table 1), which has an IHF null phenotype due to an insertion in the hip (himD) gene (9). LacZ activities in cells growing in the absence or presence of acetoacetate were monitored.…”

Section: Resultsmentioning

confidence: 99%

Interactions of the Antizyme AtoC with Regulatory Elements of the Escherichia coli atoDAEB Operon

Matta¹,

Lioliou

Kyriakidis

et al. 2007

J Bacteriol

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AtoC has a dual function as both an antizyme, the posttranslational inhibitor of polyamine biosynthetic enzymes, and the transcriptional regulator of genes involved in short-chain fatty acid catabolism (the atoDAEB operon). We have previously shown that AtoC is the response regulator of the AtoS-AtoC two-component signal transduction system that activates atoDAEB when Escherichia coli is exposed to acetoacetate. Here, we show that the same cis elements control both promoter inducibility and AtoC binding.

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Repression of the λ pcin promoter by integrative host factor

Cited by 44 publications

References 29 publications

Integration host factor is required for the activation of developmentally regulated genes in Caulobacter.

Integration host factor is required for the activation of developmentally regulated genes in Caulobacter.

HU and integration host factor function as auxiliary proteins in cleavage of phage lambda cohesive ends by terminase

Interactions of the Antizyme AtoC with Regulatory Elements of the Escherichia coli atoDAEB Operon

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