Reproducibility of Programmed-Temperature Retention Indices under Average Linear Velocity Carrier Gas Control of GC and GC–MS

Abstract

Programmed-temperature retention indices (PTRIs) are useful for the identification and quantification of chemicals by means of GC and GC/MS. To obtain reproducible PTRIs, we studied the influence of average linear velocity and column length under the constant average linear velocity control (CVC) using 55 pesticides and 33 fatty acid methyl esters. The PTRIs decreased with increasing average linear velocity, and the degree of decrease differs with the compounds. The PTRIs, however, were almost constant as long as the same average linear velocity was used even if the column length was changed. In addition, the correlation between the variation ratio in PTRI and the increase in average linear velocity was linear. Using the linear relationship between PTRIs and average linear velocities under CVC, highly reproducible PTRIs were obtained even if the capillary column length changes. Furthermore, the PTRIs determined by GC with FID, ECD, and FPD under atmospheric pressure were applied to GC/ MS under vacuum or vice versa. From these results, it was confirmed that because CVC provides reproducible retention indices, CVC is advantageous for qualitative and quantitative analysis by GC and GC/MS.

Chromatographic retention indices in identification of chemical compounds

“…They argued that the procedure turns GC RI data into a reliable, accurate tool for compound identification, even when standards are not physically present. Note that Miyagawa et al [48] recently studied the reproducibility of programmed-temperature RIs under average linear velocity carrier gas control.…”

Qualitative analysis of flavors and fragrances added to tea by using GC–MS

“…Flavors and fragrances are one of the fields where the retention index is most widely used. Among the domestic literature reports, there are many studies using GC–MS combined with retention indices for the analysis of aroma components, and this method is currently widely accepted by international academic counterparts, and most international journals also require the calculated retention indices (RI) values when the unknown aroma components are analyzed by GC–MS. Among them, calculated RI values were compared with available retention data, such as the NIST Chemistry WebBook and the English Essential Oil Database (ESO2010 Version) .…”

Nitrogen Purge Condition for Simultaneous GC/MS Measurement of Chemicals

“…Including the Pollutant Release and Transfer Register (PRTR) system, the promotion of chemical management is required internationally, and the need for environmental risk evaluation of chemicals has increased in recent years. Therefore, environmental monitoring of chemicals has become important for the evaluation of risk to humans and aquatic life, and effective simultaneous monitoring of large number of chemicals in the environment is necessary (Kadokami et al, 2009;Kameya et al, 2010;Miyagawa et al, 2011;Kondo et al, 2012;Kadokami et al, 2013).…”