Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress

Brokordt, Katherina; Pérez, Hernán; Herrera, Catalina; Gallardo, Alvaro

doi:10.3354/ab00626

Cited by 31 publications

(15 citation statements)

References 51 publications

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“…This study suggests that the induction of significant higher expressions of Hsp70 in both ovary and hepatopancreas may result in poor reproductive performance. This may due to both expression of Hsp70 and reproduction need high energy budget which consequently may affecting one another as has been described in previous study by Brokordt, Pérez, Herrera and Gallardo () and Madeira et al. ().…”

Section: Resultsmentioning

confidence: 63%

Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn, Macrobrachium rosenbergii

et al. 2017

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Using Hsp70 as a biomarker, thermal stress impinges on reproductive organs, ovary and hepatopancreas were being analyzed by determining the expression of Hsp70 mRNA inside the organs after the adult inter-molt females were subjected to thermal treatment at 35, 30 and 28°C (Control). Results showed the expression of Hsp70 mRNA under thermal treatment of 35°C after 2 hr recovery in ovary were upregulated at 2, 4, 6, 12, 24 hr and 30 days compared to control whereas in hepatopancreas under similar treatment, the expression of Hsp70 mRNA were significantly higher than control at 6, 24 hr and 30 days. Frequency of reproductive molt at 35°Cshowed the ovary of females were failed to develop and only entered common molt along three consecutive molt cycles. For 30°C thermal treatment, the expression of Hsp70 mRNA was significantly higher than control after 2 hr recovery but returned to normal afterwards until 30 days' thermal treatment. Maternal heat shock for 2 hr at 35°C were found to give significantly lower frequency of reproductive molt and longer duration of ovarian development and incubation period whereas maternal heat shock for 2 hr at 30°C gave lower frequency of reproductive molt, slower development of embryo and lower hatching success compared to untreated control. This study suggests that short and long-term thermal stress at 30 and 35°C were found to affect the induction of Hsp70 mRNA in reproductive organs of Macrobrachium rosenbergii and also influence their reproductive performance. K E Y W O R D Sheat shock protein 70, Macrobrachium rosenbergii, reproductive performance, thermal stress

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Section: Resultsmentioning

confidence: 63%

Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn, Macrobrachium rosenbergii

et al. 2017

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“…Total protein was quantified in 0.03 g (wet mass) of hemocyte pellets from each scallop following Brokordt et al (2015). Hemocytes were homogenized in 150 μL of homogenization buffer (32 mM Tris-HCl at pH 7.5, 2% SDS, 1 mM EDTA, 1 mM Pefabloc and 1 mM protease inhibitor cocktail; Sigma) and incubated for 5 min at 100°C.…”

Section: Methodsmentioning

confidence: 99%

“…Because scallops are broadcast spawners, they invest massively in reproduction to produce abundant energy-rich gametes in order to ensure fertilization success, and to allow early planktonic development without external food. In this group of bivalves, energy investment in reproduction has shown to reduce other vital processes such as swimming ability and escape response capacity (Brokordt et al, 2000a,b, 2003, 2006; Kraffe et al, 2008), and tolerance to environmental stress (Brokordt et al, 2015). In addition, post-reproduction mass mortalities have been reported for scallops and other marine bivalves (Tremblay et al, 1998; Xiao et al, 2005; Samain et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Reproduction Immunity Trade-Off in a Mollusk: Hemocyte Energy Metabolism Underlies Cellular and Molecular Immune Responses

et al. 2019

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Immune responses, as well as reproduction, are energy-hungry processes, particularly in broadcast spawners such as scallops. Thus, we aimed to explore the potential reproduction-immunity trade-off in Argopecten purpuratus, a species with great economic importance for Chile and Peru. Hemocytes, key immunological cells in mollusks, were the center of this study, where we addressed for the first time the relation between reproductive stage, hemocyte metabolic energetics and their capacity to support immune responses at cellular and molecular levels. Hemocyte metabolic capacity was assessed by their respiration rates, mitochondrial membrane potential and citrate synthase (CS) activity. Cellular immune parameters such as the number of circulating and tissue-infiltrating hemocytes and their reactive oxygen species (ROS) production capacity were considered. Molecular immune responses were examined through the transcriptional levels of two pattern recognition receptors (ApCLec and ApTLR) and two anti-microbial effectors (ferritin and big defensin). Their expressions were measured in hemocytes from immature, matured and spawned scallops under basal, and one of the following challenges: (i) in vitro, where hemocytes were challenged with the β glucan zymosan, to determine the immune potentiality under standardized conditions; or (ii) in vivo challenge, using hemocytes from scallops injected with the pathogenic bacteria Vibrio splendidus. Results indicate a post-spawning decrease in the structural components of the immune system (hemocyte number/quality) and their potential capacity of performing immune functions (with reduced ATP-producing machinery and exhaustion of energy reserves). Both in vitro and in vivo challenges demonstrate that hemocytes from immature scallops have, in most cases, the best metabolic potential (increased CS activity) and immune performances, with for example, over threefold higher ROS production and tissue-infiltration capacity than those from mature and spawned scallops after the bacterial challenge. Agreeing with cellular responses, hemocytes from immature individuals induced the highest levels of immune receptors and antimicrobial effectors after the bacterial challenge, while spawned scallops presented the lowest values. Overall, results suggest a trade-off between resource allocation in reproduction and the immune responses in A. purpuratus, with hemocyte energy metabolic capacity potentially underlying cellular and molecular immune responses. Further research would be necessary to explore regulatory mechanisms such as signaling pleiotropy which may potentially be underlying this trade-off.

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“…It furthermore depends on different types of Hsps in different organisms. It also may comprise from the induction of many other non Hsps genes (Brokordt et al, 2015;Junprung et al, 2017;Lindquist, 1986;Madeira, 2014;Ravaux et al, 2016).…”

Section: The Effect Of the Temperature On The Innate Immune System Momentioning

confidence: 99%

Innate Immune System Response Against Environmental Temperature Changes as A Dangerous Abiotic Factor

2020

Global NEST: The International Journal

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<p>Innate immune system is the first line response against environmental changes in invertebrate. It protects the animal from environmental changes such as temperature change, PH and salinity as well as pathogens such as bacteria and viruses through different biochemical pathways. In fact, the innate immune system relies on different biochemical reactions which are protecting the animal under adverse environmental circumstances. Among all of the environmental factors, temperature is a dangerous abiotic factor which affects organisms on its ecological level through infiltrating it’s the molecular and cellular levels. Invertebrate could survive from a wide range of environmental effects and possesses innate immunity as its defense systems. This review paper aimed at presenting the main innate immune pathways that are activated against the most abiotic environmental changes. We reviewed fundamental aspects of invertebrates’ defense process by focusing on the important innate immune pathways including: Pattern recognition receptors (PRRs), Antimicrobial peptide (AMP), Pro-PO activating system, Melanization Pathway, Lectin Pathway, Apoptosis Pathway, Plasma clotting protein.</p>

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Reproduction reduces HSP70 expression capacity in Argopecten purpuratus scallops subject to hypoxia and heat stress

Cited by 31 publications

References 51 publications

Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn, Macrobrachium rosenbergii

Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn, Macrobrachium rosenbergii

Reproduction Immunity Trade-Off in a Mollusk: Hemocyte Energy Metabolism Underlies Cellular and Molecular Immune Responses

Innate Immune System Response Against Environmental Temperature Changes as A Dangerous Abiotic Factor

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