Reproductive hormones in seminal plasma; it's effect on semen quality

Abstract

The aim ofthis study was to investigate the effect of reproductive hormones in seminal plasma on the quality of the spermatozoa in men seeking infertility treatment. Of the 383 volunteers recruited, 123 had normal sperm parameters and 260 had either a single or a combination of sperm abno rmalities as per WHO criteria . Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) Prolactin (PRL), Testosterone (T) and Estradiol (E2) levels in serum and semen of these men were determ in ed using an immunometri c chemi luminisance assay. Of the serum hormones, FSH, LH were significantly higher (p<O.OOI for both) and T levels were significantly lower (p< 0.01) in men with abnormal semen compared with the men with normal semen parameters. Serum PRL and E2 in both the groups did not show any significant difference. With regard to semen hormones FSH, LH and T levels in men with' normal and abnormal semen did not show any significant difference. In contrast, men with abnormal semen had significantly higher levels of semen PRL (p<O.OOI) and E2 (p<O.OI) compared with those in men with normal semen. Moreover, semen PRL showed a significant negative correlation with sperm motility (r = -D.398; p<O.OOI), morphology (r = -0.32; p<O.OI) and viability (r = -0.328; p<O.OI). Further, a positive correlation between PRLand semen volume was observed in both semen normal and abno rmal groups (r = 0.21; p<0.05). Semen E2 in the abnorma l g roup also showed a Significant negative relationship with sperm motility (r = -D.305; P < 0.01). In conclusion, in men with abnormal semen parameters both PRL and E2 in seminal plasma have a negative impact on sperm motility. It was also evident that semen PRL has a negative impact on sperm morphology and Viability.

The Effects of Lead and Cadmium on Sperm Parameters Including Sperm DNA Fragmentation of Men Investigated for Infertility

“…The study subjects were assigned in to two groups: Normozoospermics and pathozoospermics based on the sperm parameters (WHO 2010) and comparisons were made in the two groups under the following, a) exposure to environmental and occupational reproductive toxicants and semen parameters b) exposure to environmental and occupational reproductive toxicants and presence of lead and cadmium in seminal plasma and c) the presence of lead and cadmium in seminal plasma and semen parametersIn the total population there were 99 (33%) pathozoospermics and 201 normozoospermics (67%). Similar previos studies carried out in Sri Lanka in different regions report that approximately 55-60% were pathozoospermics and 40%-45% were normozoospermics (219) (220)(221). The difference in the results obtained in current study and previous studies was due to the changes resulting in lower limits of the sperm parameters in WHO reference ranges on which normozoospennics and pathozoospermics are categorised.…”

Seminal prolactin is associated with HSP90 transcript content in ejaculated spermatozoa

“…found that seminal levels of PRL did not differ between normozoospermic and teratozoospermic men. On the contrary, Wijeratna et al [ 34 ] reported that there was a significant negative relationship between seminal PRL levels and sperm morphology. In vitro incubation of human spermatozoa in the presence of PRL increases motility and decreases DNA damage in a dose-dependent manner [ 13 ] and stimulates cell metabolism [ 10 ].…”

Biochemical analysis of bovine (BosIndicus) seminal plasma

“…The seminal plasma, the liquid component of semen, consists of secretions from testes, epididymis, and the accessory sex glands, contains a variety of factors (amino acids, fatty acids, asmolytes and proteins) that influence the viability and fertilizating capacity of ejaculated spermatozoa (Mann and Lutwak-Mann, 1981;1990;Yanagimachi, 1994;Goericke et al, 2015). As seminal plasma is the immediate environment of spermatozoa, the substances in the seminal plasma could be expected to have profound effect on the quality of the sperm (Wijeranta et al, 2005). Seminal plasma was reported to be important for maintaining spermatozoa motility in bull (Baas et al, 1983).…”