Reprogramming Restores Vision in Mice by Changing DNA Methylation

Shi, Jiali; Sun, Shanshan; Wang, Yongjie; Huang, Zhihui

doi:10.1007/s12264-021-00729-1

Cited by 1 publication

(1 citation statement)

References 11 publications

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“…According to the clinical phenotype, pregnant women need to supplement FA as it can prevent various malformations of neural tubes. [45][46][47] FA also induces spinal axon regeneration via DNA methylation 26,48,49 and alleviates neuropathic pain in spinal cord injury rats. 50 However, the role of FA in PMS-induced GHS was unclear.…”

Section: Discussionmentioning

confidence: 99%

DNMT1 involved in the analgesic effect of folic acid on gastric hypersensitivity through downregulating ASIC1 in adult offspring rats with prenatal maternal stress

Wang

Zhang

et al. 2023

CNS Neurosci Ther

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Aims Gastric hypersensitivity (GHS) is a characteristic pathogenesis of functional dyspepsia (FD). DNA methyltransferase 1 (DNMT1) and acid‐sensing ion channel 1 (ASIC1) are associated with GHS induced by prenatal maternal stress (PMS). The aim of this study was to investigate the mechanism of DNMT1 mediating the analgesic effect of folic acid (FA) on PMS‐induced GHS. Methods GHS was quantified by electromyogram recordings. The expression of DNMT1, DNMT3a, DNMT3b, and ASIC1 were detected by western blot, RT‐PCR, and double‐immunofluorescence. Neuronal excitability and proton‐elicited currents of dorsal root ganglion (DRG) neurons were determined by whole‐cell patch clamp recordings. Results The expression of DNMT1, but not DNMT3a or DNMT3b, was decreased in DRGs of PMS rats. FA alleviated PMS‐induced GHS and hyperexcitability of DRG neurons. FA also increased DNMT1 and decreased ASIC1 expression and sensitivity. Intrathecal injection of DNMT1 inhibitor DC‐517 attenuated the effect of FA on GHS alleviation and ASIC1 downregulation. Overexpression of DNMT1 with lentivirus not only rescued ASIC1 upregulation and hypersensitivity, but also alleviated GHS and hyperexcitability of DRG neurons induced by PMS. Conclusions These results indicate that increased DNMT1 contributes to the analgesic effect of FA on PMS‐induced GHS by reducing ASIC1 expression and sensitivity.

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