Resazurin-Based Assay to Evaluate Cell Viability After Quantum Dot Interaction

Pereira, Maria I.A.; Monteiro, Camila A. P.; Oliveira, Weslley Felix de; Santos, Beate S.; Fontes, Adriana; Filho, Paulo E. Cabral

doi:10.1007/978-1-0716-0463-2_12

Cited by 11 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Only at 2000 ppm concentration, the cell viability decreases to ∼50%. A similar outcome has been previously reported using differently functionalized UCNPs, which showed low cytotoxicity even at higher concentrations ,, when compared with other metallic nanoparticles and quantum dots . We have previously reported a viability in excess of 80% for UCNP incubated 3 T3 derived fat cells (normal cells) .…”

Section: Resultssupporting

confidence: 87%

“…A similar outcome has been previously reported using differently functionalized UCNPs, which showed low cytotoxicity even at higher concentrations 20,24,34 when compared with other metallic nanoparticles 45 and quantum dots. 46 We have previously reported a viability in excess of 80% for UCNP incubated 3 T3 derived fat cells (normal cells). 34 This shows the potential of the UCNP-based composites for biomedical applications, where toxicity is a major limiting factor.…”

Section: Acs Appliedmentioning

confidence: 98%

See 1 more Smart Citation

Radiolabeled Human Protein-Functionalized Upconversion Nanoparticles for Multimodal Cancer Imaging

Akhtar

Chen

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

Multimodal imaging removes the intrinsic limitations of individual modalities, while the multipurpose nanoagents reduce the toxicity associated with the use of multiple contrast agents. However, limited functionality, inefficient or incompatible targeting, and unknown biodistribution are significant drawbacks for such complicated nanodesigns. We report a biofriendly pentamodal imaging agent with the synergistic dual-targeting ability (passive and active) based on fluoromagnetic NaGdF4:Yb,Er upconverting nanoparticles (UCNPs). Besides the nano-enabled passive targeting, the apo-human serum transferrin protein (Tf) conjugation actively targets the transferrin receptors (TfR) over-expressed on different cancer cells. The radiolabel, 99mTc, chelates in the Tf core increase the radiolabeling efficiency (∼95%) and stability (∼100% for 24 h), enabling efficient single-photon emission computed tomography imaging. Deep-penetrating 980 nm excited UCNPs are inherently active luminescent and photothermal agents enabling fluorescence and photothermal imaging, respectively. The Gd in UCNPs produces a T1-weighted MRI signal, and the Yb helps in computed tomography (CT) as contrast agents. A pentamodal imaging agent (UCNP@Tf-99mTc) with synergistic dual-targeting ability is thus designed. In vitro studies demonstrated receptor-mediated endocytosis and minimal cytotoxicity of the UCNP@Tf-99mTc nanoformulation (>75% cell viability at 1000 ppm). The in vivo biodistribution studies in the mice model under SPECT/CT revealed its near-ideal bioavailability and renal clearance behavior. The MRI investigation on 4 T1 tumor-bearing mice brought direct evidence of passive and synergistic passive-active targeted tumor accumulation of the nanoformulation. The in vivo multimodal tumor imaging (MRI/SPECT/thermal-camera/CT) demonstrates the potency of UCNP@Tf-99mTc as a single replacement for multiple imaging agents and a perfect candidate for the ultramodern Advanced Multimodality Image Guided Operating (AMIGO) suite.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Acs Appliedmentioning

confidence: 98%

Radiolabeled Human Protein-Functionalized Upconversion Nanoparticles for Multimodal Cancer Imaging

Akhtar

Chen

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

show abstract

“…Biocompatibility and cytotoxicity of biomaterials are routinely measured in in vitro assays in cell cultures, e.g., to assess the total metabolic activity of cells. Among these, the MTT assay 48 and the resazurin reduction assay, 49 also known as the AlamarBlue assay (BioRad Laboratories, Hercules, CA), 50 are frequently used. Both assays are based on the reductive activity of mitochondrial NAD(P)H-dependent oxidoreductase enzymes in viable cells, which reduce the yellow tetrazolium salt and blue resazurin dye to purple formazan crystals and soluble pink resorufin, respectively.…”

Section: Discussionmentioning

confidence: 99%

MXene-Assisted Ablation of Cells with a Pulsed Near-Infrared Laser

Kyrylenko

Gogotsi²,

Baginskiy³

et al. 2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Innovative therapies are urgently needed to combat cancer. Thermal ablation of tumor cells is a promising minimally invasive treatment option. Infrared light can penetrate human tissues and reach superficial malignancies. MXenes are a class of 2D materials that consist of carbides/nitrides of transition metals. The transverse surface plasmons of MXenes allow for efficient light absorption and light-to-heat conversion, making MXenes promising agents for photothermal therapy (PTT). To date, near-infrared (NIR) light lasers have been used in PTT studies explicitly in a continuous mode. We hypothesized that pulsed NIR lasers have certain advantages for the development of tailored PTT treatment targeting tumor cells. The pulsed lasers offer a wide range of controllable parameters, such as power density, duration of pulses, pulse frequency, and so on. Consequently, they can lower the total energy applied and enable the ablation of tumor cells while sparing adjacent healthy tissues. We show for the first time that a pulsed 1064 nm laser could be employed for selective ablation of cells loaded with Ti3C2T x MXene. We demonstrate both low toxicity and good biocompatibility of this MXene in vitro, as well as a favorable safety profile based on the experiments in vivo. Furthermore, we analyze the interaction of MXene with cells in several cell lines and discuss possible artifacts of commonly used cellular metabolic assays in experiments with MXenes. Overall, these studies provide a basis for the development of efficient and safe protocols for minimally invasive therapies for certain tumors.

show abstract

“…The level of reduction is proportional to the number of viable cells. Resazurin was chosen to perform a cell viability assay because it is not toxic and, therefore, cells exposed to it can remain in the culture to be used for other experimental purposes [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Different Strategies to Attenuate the Toxic Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells

et al. 2022

View full text Add to dashboard Cite

Zinc oxide nanoparticles (ZnO NPs) are one of the most used nanoparticles due to their unique physicochemical and biological properties. There is, however, a growing concern about their negative impact on male reproductive health. Therefore, in the present study, two different strategies were used to evaluate the recovery ability of spermatogonia cells from the first stage of spermatogenesis (GC-1 spg cell line) after being exposed to a cytotoxic concentration of ZnO NPs (20 µg/mL) for two different short time periods, 6 and 12 h. The first strategy was to let the GC-1 cells recover after ZnO NPs exposure in a ZnO NPs-free medium for 4 days. At this phase, cell viability assays were performed to evaluate whether this period was long enough to allow for cell recovery. Exposure to ZnO NPs for 6 h and 12 h induced a decrease in viability of 25% and 41%, respectively. However, the recovery period allowed for an increase in cell viability from 16% to 25% to values as high as 91% and 84%. These results strongly suggest that GC-1 cells recover, but not completely, given that the cell viability does not reach 100%. Additionally, the impact of a synthetic chalcone (E)-3-(2,6-dichlorophenyl)-1-(2-hydroxyphenyl)prop-2-en-1-one (1) to counteract the reproductive toxicity of ZnO NPs was investigated. Different concentrations of chalcone 1 (0–12.5 µM) were used before and during exposure of GC-1 cells to ZnO NPs to mitigate the damage induced by NPs. The protective ability of this compound was evaluated through viability assays, levels of DNA damage, and cytoskeleton dynamics (evaluating the acetylated α-tubulin and β-actin protein levels). The results indicated that the tested concentrations of chalcone 1 can attenuate the genotoxicity induced by ZnO NPs for shorter exposure periods (6 h). Chalcone 1 supplementation also increased cell viability and stabilized the microtubules. However, the antioxidant potential of this compound remains to be elucidated. In conclusion, this work addressed the main cytotoxic effects of ZnO NPs on a spermatogonia cell line and analyzed two different strategies to mitigate this damage, which represent a significant contribution to the field of male fertility.

show abstract

Resazurin-Based Assay to Evaluate Cell Viability After Quantum Dot Interaction

Cited by 11 publications

References 18 publications

Radiolabeled Human Protein-Functionalized Upconversion Nanoparticles for Multimodal Cancer Imaging

Radiolabeled Human Protein-Functionalized Upconversion Nanoparticles for Multimodal Cancer Imaging

MXene-Assisted Ablation of Cells with a Pulsed Near-Infrared Laser

Different Strategies to Attenuate the Toxic Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells

Contact Info

Product

Resources

About