Resazurin based microtitre assay and comparison of DLA and EAC cell lines in Amaranthus viridis L.

Abstract

Amaranthus viridis L. a medicinal plant belonging to family Amaranthaceae, is used for bactericidal activity to kill infectious diseases and has reported to be antioxidant and anticancer though it contains glycosides, phenols and flavanoids. The study conducted to evaluate the anticancer ability using the cell lines and antimicrobial activity using resazurin dye. The leaf extract were made by cold extraction using different solvent based on polarity. Antimicrobial activity was determined using resazurin based microtitre assay and MIC and anticancer study was conducted in DLA and EAC cell lines. The result showed ethanol and methanol extract posses antimicrobial activity (1.25-0.625 mg/mL). DLA cell line showed good percentage of activity than EAC cell line. Amaranthus viridis L. extract showed antibacterial and antifungal activity against human pathogenic strains and has got anticancer property.