Rescuing monopronucleated-derived human blastocysts: a model to study chromosomal topography and fingerprinting

Soler, Nuria; Bautista-Llácer, Rosa; Escrich, L.; Oller, Andrea; Grau, N.; Tena, Raquel; Insua, María Fernanda; Ferrer, Paloma; Escribá, Marı́a-José; Vendrell, Xavier

doi:10.1016/j.fertnstert.2021.03.038

Cited by 6 publications

(3 citation statements)

References 73 publications

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“…Congenital malformations at birth are not enough to detect possible anomalies in the children born after the transfer of those embryos. However, a significant proportion of euploid 1PN blastocysts will have maternal uniparental disomy ( 28 ). Beyond PGT testing for euploidy, biparental diploidy verification becomes the next direction for exploration, as shown by Soler et al.…”

Section: Discussionmentioning

confidence: 99%

“…Beyond PGT testing for euploidy, biparental diploidy verification becomes the next direction for exploration, as shown by Soler et al. ( 28 ). Chromosomal testing for male and female embryos before IVF cycles is routine.…”

Section: Discussionmentioning

confidence: 99%

“…However, a significant proportion of euploid 1PN blastocysts will have maternal uniparental disomy (28). Beyond PGT testing for euploidy, biparental diploidy verification becomes the next direction for exploration, as shown by Soler et al (28). Chromosomal testing for male and female embryos before IVF cycles is routine.…”

Section: Discussionmentioning

confidence: 99%

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Developmental potential of non- and mono-pronuclear zygotes and associated clinical outcomes in IVF cycles

Zhu,

Dong,

Zhu

et al. 2024

Front. Endocrinol.

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PurposeThis study aims to evaluate the developmental potential of 0PN, 1PN, and 2PN zygotes in IVF cycles and compare their clinical outcomes.MethodsWe conducted a retrospective cohort study involving IVF patients. Blastocyst formation rates were assessed with 0PN, 1PN, and 2PN zygotes. Subsequently, we collected clinical outcome data following the transfer of these zygotes.ResultsThe overall blastulation rate was similar between 0PN (29.6%) and 2PN (32.1%) zygotes, but 1PN zygotes exhibited a significantly lower blastulation rate (17.0%) compared to both 0PN and 2PN zygotes. Similarly, the overall rate of good-quality blastulation was comparable between 0PN (15.3%) and 2PN (17.5%) zygotes, while 1PN zygotes showed a significantly lower rate (7.0%) compared to both 0PN and 2PN. Clinical pregnancy, ectopic pregnancy, implantation, and live birth rates were similar among single blastocyst frozen embryo transfers (FET) of 0PN, 1PN, and 2PN. Additionally, no significant differences were observed between single- and double-blastocyst FET of 0PN and 2PN.ConclusionsOur findings suggest that 0PN and 2PN zygotes have comparable developmental potential, while 1PN embryos exhibit lower developmental potential. Blastocyst FET outcomes appear similar among 0PN, 1PN, and 2PN zygotes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Developmental potential of non- and mono-pronuclear zygotes and associated clinical outcomes in IVF cycles

Zhu,

Dong,

Zhu

et al. 2024

Front. Endocrinol.

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The utility of human two plus one small pronucleated zygotes (2.1PN) based on clinical outcomes and the focused ploidy analysis

Hattori,

Okuyama,

Ashikawa

et al. 2024

J Assist Reprod Genet

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Purpose Are human embryos arising from two plus one small pronucleated zygotes, called 2.1 pronuclei (PN), clinically useful? Methods In a retrospective embryo cohort study and prospective experimental study, a total of 287 cycles in which at least one 2.1PN was identified in the fertilization check were included. Embryonic development and clinical outcome were compared for the 1395 2PN zygotes and 304 2.1PN zygotes that were siblings. All embryos were individually cultured in time-lapse systems. Twenty-five 2.1PN-derived blastocysts, donated for research, were used in focused single-nucleotide variant ploidy analysis to identify the distribution pattern of heterozygosity. Results The average diameter of PN was 24.9 ± 2.4 µm for large PN and 10.2 ± 2.4 µm for small PN; 79.9% of small PN was derived from female pronuclei. Blastocyst formation rate and good-quality blastocyst rate were significantly lower with 2.1PN embryos than with 2PN embryos (40.0% vs. 57.7%, 21.4% vs. 33.5%, respectively). A total of 13 embryos derived from 2.1PN were transferred, and three healthy babies were born. In ploidy constitutions of trophectoderm (TE), 2.1PN-derived blastocyst TE was shown to be mostly diploid (95.8%, 23/24), and only one blastocyst showed triploid. Conclusions It was suggested that 2.1PN embryos have lower embryonic developmental potential than 2PN embryos, but most of the 2.1PN were diploid, indicating that they are likely to be clinically usable. It is recommended to perform embryo transfer following a combination of PGT-A and ploidy analysis.

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Micronuclei in 2-cell embryos show higher blastocyst formation rates on human embryonic development

Yao,

Wang,

Liu

et al. 2024

European Journal of Obstetrics & Gynecology and Reproductiv

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Rescuing monopronucleated-derived human blastocysts: a model to study chromosomal topography and fingerprinting

Cited by 6 publications

References 73 publications

Developmental potential of non- and mono-pronuclear zygotes and associated clinical outcomes in IVF cycles

Developmental potential of non- and mono-pronuclear zygotes and associated clinical outcomes in IVF cycles

The utility of human two plus one small pronucleated zygotes (2.1PN) based on clinical outcomes and the focused ploidy analysis

Micronuclei in 2-cell embryos show higher blastocyst formation rates on human embryonic development

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