The study of crude oil oxidation characteristics is fundamental to the design of ignition in situ combustion. Experimentation is the most crucial method for studying the oxidation characteristics of crude oil. Aiming to address the challenges posed by high temperature, high pressure, and rapid temperature changes during the combustion of crude oil, a dynamic simulation system for high-temperature and high-pressure ignition is designed. In order to study the oxidation characteristics of the crude oil ignition process, we conducted experiments using a high-temperature and high-pressure dynamic ignition simulation device. The experiments focused on determining the ignition point of crude oil under different pressure conditions, oil–water ratios, heating rates, gas injection rates, and other relevant characteristics. The kinetic model for the oxidation process of crude oil ignition was established. The kinetic parameters were calculated for different ignition conditions and the apparent activation energy for each oxidation stage was determined. Additionally, the stability of in situ combustion was evaluated under various ignition parameters. The results show that the Arrhenius curves for crude oil exhibit noticeable differences in the HTO (high-temperature oxidation) and LTO (low-temperature oxidation) regions. The curves demonstrate good linearity in the HTO region, with correlation coefficients exceeding 0.9. Moreover, the apparent activation energies in the HTO region range from 8.01 to 26.7 kJ/mol. The apparent activation energies and finger front factors were calculated for the HTO stage under different pressure conditions. The results showed that, as the pressure increased, the autoignition point, inflection point temperature, and apparent activation energy of the crude oil decreased. This suggests that increasing the pressure can enhance the HTO of the crude oil. The spontaneous ignition point of the crude oil exhibited an upward trend as the heating rate increased. Additionally, the maximum temperature during the combustion process generally increased with the heating rate, reaching a maximum temperature of 453.1 °C. The tests demonstrated that the simulation system is capable of real-time monitoring and recording of oxidation parameters during the combustion process of crude oil. This system can provide essential data for project implementation and numerical simulation.