Research Article Improved methods for storing and extracting DNA from <i>Ilex</i> <i>paraguariensis</i> (Aquifoliaceae) tissue samples

Abstract

mate leaves can be stored on silica gel or in CTAB buffer for up to 90 days at room temperature without reduction in DNA quality. Samples stored in CTAB buffer can be refrigerated at 4ºC to minimize oxidation of phenolic compounds. The improved methods for sample storage and DNA extraction with CTAB maintain quantity and integrity for conducting molecular studies of mate.