Research Article Resistance to orange rust associated with the G1 molecular marker in parents of Brazilian RB sugarcane varieties

Borella, J.; Brasileiro, B.P; Azeredo, Amaro Afonso Campos de; Ruaro, L.; Oliveira, Rubens Alves de; Filho, João Carlos Bespalhok

doi:10.4238/gmr18980

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…On the other hand, Fier et al [ 32 ] observed high efficiency in predicting resistance using the G1 marker (71.43%) when evaluating 24 Brazilian commercial cultivars. Borella et al [ 73 ] evaluated 63 sugarcane progenitors preserved in the RIDESA germplasm bank and found that the molecular marker G1 was 71% accurate in predicting the resistance phenotype and thus could be used to characterize germplasm. There are different ways to classify resistant genotypes using a rating scale, and there is some evidence that more aggressive isolates or races of P .…”

Section: Discussionmentioning

confidence: 99%

Molecular evaluation and phenotypic screening of brown and orange rust in Saccharum germplasm

Oliveira,

Barreto,

Balsalobre

et al. 2024

PLoS ONE

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Brazil is the largest global producer of sugarcane and plays a significant role—supplier of sugar and bioethanol. However, diseases such as brown and orange rust cause substantial yield reductions and economic losses, due decrease photosynthesis and biomass in susceptible cultivars. Molecular markers associated with resistance genes, such as Bru1 (brown rust) and G1 (orange rust), could aid in predicting resistant genotypes. In this study, we sought to associate the phenotypic response of 300 sugarcane accessions with the genotypic response of Bru1 and G1 markers. The field trials were conducted in a randomized block design, and five six-month-old plants per plot were evaluated under natural disease conditions. Genotypic information about the presence or absence of Bru1 (haplotype 1) and G1 gene was obtained after extraction of genomic DNA and conventional PCR. Of the total accessions evaluated, 60.3% (181) showed resistance to brown rust in the field, and of these, 70.7% (128) had the Bru1 gene present. Considering the field-resistant accessions obtained from Brazilian breeding programs (116), the Bru1 was present in 77,6% of these accessions. While alternative resistance sources may exist, Bru1 likely confers enduring genetic resistance in current Brazilian cultivars. Regarding the phenotypic reaction to orange rust, the majority of accessions, 96.3% (288), were field resistant, and of these, 52.7% (152) carried the G1 marker. Although less efficient for predicting resistance when compared to Bru1, the G1 marker could be part of a quantitative approach when new orange rust resistance genes are described. Therefore, these findings showed the importance of Bru1 molecular markers for the early selection of resistant genotypes to brown rust by genetic breeding programs.

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Section: Discussionmentioning

confidence: 99%

Molecular evaluation and phenotypic screening of brown and orange rust in Saccharum germplasm

Oliveira,

Barreto,

Balsalobre

et al. 2024

PLoS ONE

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“…Currently available diagnostic markers revealing sources of resistance to sugarcane diseases are useful for studying the frequency and distribution of resistance sources present in the germplasm, even in the absence of disease conditions in the field. They have been widely applied to identify parents carrying specific resistance sources [7][8][9][10][11]. Additionally, a well-planned production system requires not just one but several different resistance sources coexisting in the cultivated area to extend the useful life of such resistance genes.…”

Section: Molecular Markers In the Breeding Programmentioning

confidence: 99%

Biotechnological Strategies Adopted for Sugarcane Disease Management in Tucumán, Argentina

Racedo,

Noguera,

Castagnaro

et al. 2023

Plants

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Sugarcane diseases can be controlled by an integrated management approach where biotechnological tools can successfully contribute. The Obispo Colombres Agroindustrial Experimental Station (EEAOC) in Tucumán (Argentina’s main sugarcane producer) has successfully implemented multiple strategies that greatly enhance the productivity of sugarcane fields. The local breeding program develops resistant varieties by applying molecular markers to reveal the presence of Bru1 gene for brown rust resistance throughout the EEAOC germplasm collection. In addition, SNP alleles linked to novel sources of resistance were identified following a selective genotyping strategy. Another strategy is the implementation of a seed cane sanitation project using hydrothermal therapy, an in vitro culture technique, molecular diagnosis of diseases, and bionanoparticles. As a result, the incidence of systemic diseases has significantly decreased in the production fields. More recently, the use of biological products has shown to be effective for disease control in EEAOC varieties. In summary, several biotechnological strategies including molecular markers associated with resistant sources, in vitro culture of apical meristems, molecular diagnostic techniques, and the use of bioproducts are being successfully used for the sustainable management of sugarcane diseases in Tucumán, Argentina.

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Research Article Resistance to orange rust associated with the G1 molecular marker in parents of Brazilian RB sugarcane varieties

Cited by 2 publications

References 27 publications

Molecular evaluation and phenotypic screening of brown and orange rust in Saccharum germplasm

Molecular evaluation and phenotypic screening of brown and orange rust in Saccharum germplasm

Biotechnological Strategies Adopted for Sugarcane Disease Management in Tucumán, Argentina

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