Research Article Whole Genome Resequencing of Jordanian Awassi Rams (&lt;i&gt;Ovis&lt;/i&gt; &lt;i&gt;aries&lt;/i&gt;) Using Hiseq Sequencing Technology: The First Step Towards Sheep Genomic Selection

Haddad, Nizar; Migdadi, Hussein M.; Al-Atiyat, Raed M.; Jawasreh, Khaleel; Awabdeh, Sami; Obeidat, Wisam; Alomari, R.; Aldamra, M.; Ababneh, H.; Tabbaa, M. J.; Brake, Mohammad; Farooq, Muhammad

doi:10.4238/gmr18577

Cited by 3 publications

(5 citation statements)

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“…The CNV results listed in Table ( 5); Over 72% of the CNV were downregulated. Recently, the whole-genome resequencing of Jordanian Awassi Ram (Ovis aries) using Hiseq sequencing technology was reported (Haddad et al, 2020). The report considered this research as the first step towards sheep genomic selection of Awassi ram.…”

Section: Structure and Copy Number Variation Detection And Annotationmentioning

confidence: 99%

“…The first whole-genome resequencing of Jordanian Awassi ram was published (Haddad et al, 2020), which reported the availability of wide genome variability (e.g., SNPs, InDel, SV, CNV) that could be helpful in the signature's detection of selection to reveal new insight into the mechanism of advanced breeding, artificial selection and genes associated essential traits. Genetic studies on Jordanian Awassi revealed genetic variability among and within breeds (Al-Atiyat et al, 2014;, genetic parameters related to growth performance, carcass characteristics and meat quality (Jawasreh et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

Migdadi

Haddad

Alomari

et al. 2021

IJAR

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Background: Jordanian Awassi sheep (Ovis aries) is the dominant fat tail sheep breed that appeals to customers because of its various production systems, including fiber, meat and milk. This report is the first whole ewe genome sequence (WGS) of O. aries submitted in the NCBI database from Jordan. Methods: 64 Paired-end sequencing libraries were constructed and subjected to Illumina Hiseq 2500 sequencing system. High-quality reads were aligned against the reference sheep genome and detecting comprehensive sources (SNPs, InDels, SV, CNVs) of genetic variations. We have deposited data sequences at the NCBI under SRA (sequence reads archives) under the accession numbers SRR11128863, PRJNA574879. Result: Genome resequencing of Jordanian Awassi ewe was carried out with approximately 93.88 Gb with a mapping rate and effective mapping depths were 99.28% and 36.32. Around 19 million SNPs, 3,6 million InDels, 35,180 Structure variation and 13,524 copy number variation among the Jordanian ewe genome were detected. This wide range of genetic variation provides a framework for further genetic studies that will help understand the molecular basis underlying phenotypic variation of economically important traits in sheep and improve intrinsic defects in domestic sheep breeds.

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Section: Structure and Copy Number Variation Detection And Annotationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

Migdadi

Haddad

Alomari

et al. 2021

IJAR

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“…Recently, we sequenced the whole genome of male and female Awassi-Jo (Haddad et al. 2020 ). The whole genomes of both male and female Awassi-Jo were sequenced and the ram genome was published (Haddad et al.…”

mentioning

confidence: 99%

“…In Jordan, Awassi-Jo sheep (Ovis aries) is the dominant fat tail breed, that has many unique characteristics, including acceptable performance under harsh conditions and preferred meat quality by Mediterranean consumers. Recently, we sequenced the whole genome of male and female Awassi-Jo (Haddad et al 2020). The whole genomes of both male and female Awassi-Jo were sequenced and the ram genome was published (Haddad et al 2020).…”

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confidence: 99%

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Complete mitochondrial genome sequence of Awassi-Jo sheep breed (Ovis aries) in Jordan

Brake

Migdadi

Sadder

et al. 2021

Mitochondrial DNA Part B

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Using high-throughput sequencing technology, the complete mitochondrial genome of Awassi-Jo breed ( Ovis aries ) was decoded. Mitochondrial genome was 16,617 bp in length. The genome contained 37 genes (13 protein-coding, 22 tRNA, and 2 rRNA) and a control region (D-loop region). The genes were encoded on the H-strand, except for the ND6 gene and 8 tRNA genes, which were encoded on the L-strand. The GC content is 38.9%. Phylogenetic analysis was performed to compare Awassi-Jo with other sheep breeds. The phylogenetic tree showed that Awassi-Jo diverged earlier than related breeds (Turkey, Italy, Germany, and Netherland) with a common ancestor in haplogroup HB. The results revealed the importance of mitochondrial data in studying sheep evolution and domestication.

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Growth performance and carcass characteristics of fattening Awassi lambs fed willow silage

Awabdeh

Sweidan

Landau

2022

Small Ruminant Research

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Research Article Whole Genome Resequencing of Jordanian Awassi Rams (<i>Ovis</i> <i>aries</i>) Using Hiseq Sequencing Technology: The First Step Towards Sheep Genomic Selection

Cited by 3 publications

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Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

Complete mitochondrial genome sequence of Awassi-Jo sheep breed (Ovis aries) in Jordan

Growth performance and carcass characteristics of fattening Awassi lambs fed willow silage

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Research Article Whole Genome Resequencing of Jordanian Awassi Rams (<i>Ovis</i> <i>aries</i>) Using Hiseq Sequencing Technology: The First Step Towards Sheep Genomic Selection

Cited by 3 publications

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​Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

​Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

Complete mitochondrial genome sequence of Awassi-Jo sheep breed (Ovis aries) in Jordan

Growth performance and carcass characteristics of fattening Awassi lambs fed willow silage

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Product

Resources

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Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection