Objectives: identify the interventions/activities nurses develop at a Chemotherapy Center (CTC) using standardized language and validate their contents. Method: data triangulation was used through the combination of three information sources: semistructured interview, document analysis and questionnaire. The instrument, constructed in accordance with the Nursing Interventions Classification (NIC) taxonomy, was submitted to content validation through meetings with the participants. Results: Thirty-five interventions and 48 activities were mapped and validated, organized in five domains (physiological: basic and physiological: complex, behavioral, safety and health system) and 11 classes. Conclusion: The identification of nurses' interventions/activities at CTC supports the determination of the time consumed and permits measuring the workload. It also helps to define these professionals' role, which permits the redesign of the work process and optimizes productivity.