Research on diagnostic system integrated nucleic acid extraction and detection

Meng, Xiangkun; Jin, Guangyong; Wang, Zhe; Gong, Ping; Duanmu, Luyang

doi:10.1117/12.2584970

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with the ordinary PCR method, the integrated molecular diagnostic system has multiple advantages: it is not necessary to match the equipment such as nucleic acid extraction instruments, PCR instruments, and other equipment, and only one integrated instrument can be detected; the operator simply needs to add the sample to the kit and insert it into the instrument for testing, without requiring any additional steps during the testing process, thereby optimizing time and efort efciency; fully closed automated experimental process can avoid sample crosscontamination and environmental pollution to maximize the protection of the operator's safety. Nowadays, many organizations are actively carrying out the development and research of fully automated and integrated molecular diagnostic systems [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus

Ma,

Zhu,

Jiang

et al. 2024

International Journal of Analytical Chemistry

View full text Add to dashboard Cite

Objective. A sensitive and specific multiplex fluorescence rapid detection method was established for simultaneous detection of SARS-CoV-2, influenza A virus, and influenza B virus in a self-made device within 30 min, with a minimum detection limit of 200 copies/mL. Methods. Based on the genome sequences of SARS-CoV-2, influenza A virus (FluA), and influenza B virus (FluB) with reference to the Chinese Center for Disease Control and Prevention and related literature, specific primers were designed, and a multiplex fluorescent PCR system was established. The simultaneous and rapid detection of SARS-CoV-2, FluA, and FluB was achieved by optimizing the concentrations of Taq DNA polymerase as well as primers, probes, and Mg2+. The minimum detection limits of the nucleic acid rapid detection system for SARS-CoV-2, FluA, and FluB were evaluated. Results. By optimizing the amplification system, the N enzyme with the best amplification performance was selected, and the optimal concentration of Mg2+ in the multiamplification system was 3 mmol/L; the final concentrations of SARS-CoV-2 NP probe and primer were 0.15 μmol/L and 0.2 μmol/L, respectively; the final concentrations of SARS-CoV-2 ORF probe and primer were both 0.15 μmol/L; the final concentrations of FluA probe and primer were 0.2 μmol/L and 0.3 μmol/L, respectively; the final concentrations of FluB probe and primer were 0.15 μmol/L and 0.25 μmol/L, respectively. Conclusion. A multiplex real-time quantitative fluorescence RT-PCR system for three respiratory viruses of SARS-CoV-2, FluA, and FluB was established with a high amplification efficiency and sensitivity reaching 200 copies/mL for all samples. Combined with the automated microfluidic nucleic acid detection system, the system can achieve rapid detection in 30 minutes.

show abstract

Section: Introductionmentioning

confidence: 99%