Research on Photoinduction-Based Technology for Trapping Asian Longhorned Beetle (Anoplophora glabripennis (Motschulsky, 1853) (Coleoptera: Cerambycidae)

Abstract

Light traps play a crucial role in monitoring pest populations. However, the phototactic behavior of adult Asian longhorned beetle (ALB) remains enigmatic. To provide a theoretical foundation to select the suitable light emitting diode (LED)-based light sources used for monitoring ALB, we compared the effect of exposure time on the phototactic response rates of adults at wavelengths of 365 nm, 420 nm, 435 nm, and 515 nm, and found that the phototactic rate increased gradually when the exposure time was prolonged, but there was no significant difference between different exposure times. We evaluated the effect of diel rhythm and found the highest phototactic rate at night (0:00–2:00) under 420 nm and 435 nm illumination (74–82%). Finally, we determined the phototactic behavioral response of adults to 14 different wavelengths and found both females and males showed a preference for violet wavelengths (420 nm and 435 nm). Furthermore, the effect of the light intensity experiments showed that there were no significant differences in the trapping rate between different light intensities at 120 min exposure time. Our findings demonstrate that ALB is a positively phototactic insect, showing that 420 nm and 435 nm are the most suitable wavelengths for attracting adults.

Integrated behavior and transcriptomic analysis provide valuable insights into the response mechanisms of Dastarcus helophoroides Fairmaire to light exposure

“…Previous studies have shown that Coleoptera beetles display a preference for UV, violet and green wavelength spectra (350-440 and 500-560 nm) (Kim et al, 2019;Van der Kooi et al, 2021). Using the same behavioral response chamber, both females and males A. glabripennis displayed higher phototactic responses under illumination with 420 and 435 nm wavelengths (violet and blue) at night, with an approximately 74%-82% trapping rate (Jiang et al, 2023a). UV light traps were more attractive to Arhopalus ferus and Prionoplus reticularis (longhorn beetles) than their adjacent two yellow lights .…”

“…To observe the phototactic behavior of D. helophoroides in the different conditions, a light-dark alternative apparatus with an opaque black acrylic board was self-designed based on a previous study (Figures 1A, B) (Jiang et al, 2023a). The apparatus comprised two test chambers (L = 50 cm × H = 30 cm × W = 30 cm) at right angles to each other, which provided an activity space for beetles to respond to different wavelength LED lights, and an activity chamber (L = 30 cm × H = 30 cm × W = 30 cm), which provided adequate space for insect movement.…”

“…UV-black-light-blue light traps captured more Cerambycid beetles (Arhopaus ferus) than yellow light traps . UV (365 nm) and violet light (420 and 435 nm) are the most suitable wavelengths for attracting adult A. glabripennis at night (Jiang et al, 2023a). Therefore, in order to decrease the trapping rate of natural enemy (D. helophoroides) in the light traps, it is of great significance to reveal the response mechanism of D. helophoroides adults for the UV and visible lights.…”

Investigating the Influence of Varied Light-Emitting Diode (LED) Wavelengths on Phototactic Behavior and Opsin Genes in Vespinae

“…The phototaxis monitoring system ( Figure 1 ) was designed based on Huang et al (2023) and Jiang et al (2023) [ 37 , 38 ]. It included a phototactic reaction chamber (light zone), a static chamber, and a dark chamber (dark zone).…”