Figuring out the thermal response and damage features of fabricated steel structures under fire conduces to enhancing the fire resistance of steel structures and lowering the probability of fire accidents. Available methods are able to simulate complex fire situations, but their accuracy is affected by the adopted models and calculation methods. For this reason, this paper researched the thermal response and damage features of fabricated steel structures under fire. At first, the thermal response of fabricated steel structures under fire was analyzed, the ultimate bearing capacity and the fire resistance was calculated and checked. Then, the evolution of fatigue damage of fabricated steel structures under fire was analyzed, and the fatigue damage evolution method and its flow were given. At last, combining with actual cases, the thermal response and damage features of fabricated steel structures under fire were examined experimentally, and the results verified the validity of the proposed analysis method.