Research Progress of the Application of Hypothermia in the Eye

Xi, Lei

doi:10.1155/2020/3897168

Cited by 8 publications

(7 citation statements)

References 97 publications

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“…Recent reviews have linked the experimental data obtained in animals and the potential applications of hypothermia for preventing neural damage, including retinal injury, in human newborns (Albrecht et al, 2019;Xi 2020). Interestingly, many native cultures around the world practice dipping newborns in cold water soon after birth (Connell-Szasz 1988;Degefie et al, 2014;Sacks et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

et al. 2021

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Perinatal asphyxia (PA) can cause retinopathy and different degrees of visual loss, including total blindness. In a rat model of PA, we have previously shown a protective effect of hypothermia on the retina when applied simultaneously with the hypoxic insult. In the present work, we evaluated the possible protective effect of hypothermia on the retina of PA rats when applied immediately after delivery. Four experimental groups were studied: Rats born naturally as controls (CTL), animals that were exposed to PA for 20 min at 37°C (PA), animals exposed to PA for 20 min at 15°C (HYP), and animals that were exposed to PA for 20 min at 37°C and, immediately after birth, kept for 15 min at 8°C (HYP-PA). To evaluate the integrity of the visual pathway, animals were subjected to electroretinography at 45 days of age. Molecular (real time PCR) and histological (immunohistochemistry, immunofluorescence, TUNEL assay) techniques were applied to the eyes of all experimental groups collected at 6, 12, 24, and 48 h, and 6 days after birth. PA resulted in a significant reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram. All animals treated with hypothermia had a significant correction of the a-wave and OP, but the b-wave was fully corrected in the HYP group but only partially in the HYP-PA group. The number of TUNEL-positive cells increased sharply in the ganglion cell layer of the PA animals and this increase was significantly prevented by both hypothermia treatments. Expression of the cold-shock proteins, cold-inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3), was undetectable in retinas of the CTL and PA groups, but they were highly expressed in ganglion neurons and cells of the inner nuclear layer of the HYP and HYP-PA groups. In conclusion, our results suggest that a post-partum hypothermic shock could represent a useful and affordable method to prevent asphyxia-related vision disabling sequelae.

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Section: Discussionmentioning

confidence: 99%

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

et al. 2021

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“…While induction of systemic hypothermia can be associated with significant adverse effects such as shivering, hypotension, cardiac arrhythmias, pneumonia, and thrombocytopenia, locally induced ocular hypothermia could prevent these. 88 In the eye and retina, localized hypothermia can be achieved during vitrectomy using hypothermic saline at the desired temperature; one can use the infusion fluid during vitrectomy to achieve this hypothermia if a known pre-operative temperature target is determined. We have called this “Hypothermic Vitrectomy”.…”

Section: Non-pharmacologic Neuroprotective Strategies For Craomentioning

confidence: 99%

Section: Non-pharmacologic Neuroprotective Strategies For Craomentioning

confidence: 99%

Neuroprotection for Nonarteritic Central Retinal Artery Occlusion: Lessons from Acute Ischemic Stroke

Okonkwo¹,

Agweye²,

Akanbi³

2023

OPTH

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Nonarteritic central retinal artery occlusion (NA-CRAO) is a variant of acute ischemic stroke (AIS) and is a cause of sudden severe loss of vision. There are guidelines by the American Heart Association and the American Stroke Association for the care of CRAO patients. This review explores the basis of retinal neuroprotection for CRAO and its potential for improving the outcome of NA-CRAO. Recently, there have been significant advances in research into the use of neuroprotection to treat retinal diseases, including retinal detachment, age-related macular degeneration, and inherited retinal diseases. Also, neuroprotective research in AIS has been extensive, and newer drugs tested, including Uric acid, Nerinetide, and Otaplimastat, with promising results. Progress in cerebral neuroprotection after AIS offers hope for retinal neuroprotection after CRAO; and a possibility of extrapolating research findings from AIS into CRAO. Combining neuroprotection and thrombolysis can extend the therapeutic window for NA-CRAO treatment and potentially improve outcomes. Experimented neuroprotection for CRAO includes Angiopoietin (Comp Ang1), KUS 121, Gene therapy (XIAP), and hypothermia. Efforts in the field of neuroprotection for NA-CRAO should focus on better imaging to delineate the penumbra after an acute episode of NA-CRAO (using a combination of high-definition optical coherence angiography and electrophysiology). Also, research should explore details of pathophysiologic mechanisms involved in NA-CRAO, allowing for further neuroprotective intervention, and closing the gap between preclinical and clinical neuroprotection.

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“…Currently, three forms of preservation of corneal grafts are used – organ culture solution (28–37°C), hypothermia (2–8°C), and cryopreservation (4°C). 1 Of these, hypothermia is most frequently used in the United States, while European eye banks more frequently use organ culture. Organ culture, in which tissue is incubated in a medium supplemented with fetal calf serum, antibiotics, and antimycotics, has been associated with longer viable storage times than hypothermia.…”

Section: Introductionmentioning

confidence: 99%

“…Organ culture, in which tissue is incubated in a medium supplemented with fetal calf serum, antibiotics, and antimycotics, has been associated with longer viable storage times than hypothermia. 1 However, surgeons and eye banks have become judicious with the number of days a corneal graft can be used after preservation, with many preferring not to exceed seven days when stored via hypothermia. 2 Despite this, many recent studies have found few significant associations between the preservation characteristics of the graft and corneal transplantation outcomes.…”

Section: Introductionmentioning

confidence: 99%

Impact of Donor, Recipient, and Graft Characteristics on Corneal Transplantation Outcomes

Shah¹,

Hasan²,

Winokur³

et al. 2023

OPTH

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Purpose Although several donor, recipient, and graft characteristics have been studied in relation to corneal transplantation outcomes, no study to our knowledge has assessed the impact of donor cooling times on postoperative outcomes longitudinally. With only one corneal graft available for every 70 needed worldwide, this study seeks to identify any factors that could alleviate this shortage. Methods Patients undergoing corneal transplantation at the Manhattan Eye, Ear & Throat Hospital over a 2-year period were retrospectively studied. Study metrics included age, diabetic history, hypertensive history, endothelial cell density, death-to-preservation time (DTP), death-to-cooling time (DTC), and time-in-preservation (TIP). Postoperative transplantation outcomes, including best corrected visual acuity (BCVA) at 6- and 12-month follow-up visits, need for re-bubbling, and need for re-grafting, were assessed. Unadjusted univariate and adjusted multivariate binary logistic regressions were performed to determine the association of cooling and preservation parameters with corneal transplantation outcomes. Results Among 111 transplants, our adjusted model found that DTC ≥4 hours was associated with significantly worse BCVA, but only at 6-month postoperative follow-up (odds ratio [OR]: 0.234; 95% confidence interval [CI]: 0.073–0.747; p = 0.014). By 12-month follow-up, DTC > 4 hours was no longer associated with BCVA in a statistically significant manner (OR: 0.472; 95% CI: 0.135–1.653; p = 0.240). A similar trend was found at a DTC cutoff of ≥3 hours. None of the other studied parameters, including DTP, TIP, donor age, or medical history were significantly correlated with transplantation outcomes. Conclusion Longer DTC or DTP did not have a statistically significant effect on corneal graft outcomes after one year, though short-term outcomes were improved in donor tissues with DTC below four hours. None of the other studied variables correlated with transplantation outcomes. Given the global shortage of corneal tissue, these findings should be considered when determining suitability for transplantation.

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Research Progress of the Application of Hypothermia in the Eye

Cited by 8 publications

References 97 publications

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats

Neuroprotection for Nonarteritic Central Retinal Artery Occlusion: Lessons from Acute Ischemic Stroke

Impact of Donor, Recipient, and Graft Characteristics on Corneal Transplantation Outcomes

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