Research Progress of Zebrafish Model in Aquatic Ecotoxicology

Abstract

In recent years, with the aggravation of environmental pollution, the study of aquatic ecotoxicology has become a hot topic. Some aquatic organisms, such as large fleas, toads, and zebrafish, have been developed and applied as model organisms. They have been increasingly used to study the bioaccumulation and toxicity of environmental pollutants due to the advantages of their small size, easy reproduction, short life cycle, low maintenance cost, and ability to combine genetic, cellular, and whole organism endpoints. This review summarizes the methods of zebrafish, as a model in aquatic ecotoxicology, using its developmental toxicity, biomarkers, genomics, and phenotype analysis to evaluate the toxicity of environmental pollutants, providing a reference value for researchers to select model animals for aquatic ecotoxicology research.

Green Chromatographic Methods and in Vitro Pharmacological Analysis of Varronia curassavica Leaf Derivatives

“…The zebrafish model has been used in biological analysis, such as in new drug screening, because their genome is 87 % similar to that of human. [22][23][24] Zebrafish has proved to be a useful model for evaluating the toxicity of plant extracts and their bioactive compounds. [25] In the present study, we evaluated the acute toxicity of 100 % EtOH and 70 % EtOH extracts in the embryonic development of Zebrafish.…”

“…Based on the sustainable approach employed in the development of chromatographic methods for the secondary metabolites of V. curassavica , we evaluated the toxicity of V. curassavica extracts through an alternative method using Zebrafish instead of mammalian species. The zebrafish model has been used in biological analysis, such as in new drug screening, because their genome is 87 % similar to that of human [22–24] …”

The Numbers of Animals Used in Mexico for Scientific and Educational Purposes

“…14 Furthermore, over recent years there has been an increase in the study of aquatic ecotoxicology, in which the zebrafish has been widely used as a study model. 15 This type of research is carried out at all stages of zebrafish development — i.e. in embryos, juveniles and adults; 15 zebrafish embryos are typically used for developmental toxicity tests.…”

“…15 This type of research is carried out at all stages of zebrafish development — i.e. in embryos, juveniles and adults; 15 zebrafish embryos are typically used for developmental toxicity tests. 15 The notable increase in the number of scientific publications that specifically mention the use of the zebrafish as a biological model, illustrates the extensive rise in their use.…”

“…in embryos, juveniles and adults; 15 zebrafish embryos are typically used for developmental toxicity tests. 15 The notable increase in the number of scientific publications that specifically mention the use of the zebrafish as a biological model, illustrates the extensive rise in their use. In 2015, there were 2774 such publications, while in 2022 there were a total of 4045 publications.…”