Research progress on microRNA in gout

Xie, Jing; He, Cuixia; Su, Yue; Ding, Yulong; Zhu, Xiaodong; Xu, Yuanyuan; Ding, Jiaxiang; Zhou, Huan‐Xiang; Wang, Hongju

doi:10.3389/fphar.2022.981799

Cited by 4 publications

(4 citation statements)

References 101 publications

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“…Studies have shown that some miRNAs are involved in both hyperuricemia and gout ( 22 , 67 ). For example, the expression of miR-146a was significantly increased in patients with gout and involved in both NLRP3 and MyD88/NF-κB pathways ( 68 ).…”

Section: Discussionmentioning

confidence: 99%

Characterizations of microRNAs involved in the molecular mechanisms underlying the therapeutic effects of noni (Morinda citrifolia L.) fruit juice on hyperuricemia in mice

Liu

Wang

et al. 2023

Front. Nutr.

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BackgroundHyperuricemia is generally defined as the high level of serum uric acid and is well known as an important risk factor for the development of various medical disorders. However, the medicinal treatment of hyperuricemia is frequently associated with multiple side-effects.MethodsThe therapeutic effect of noni (Morinda citrifolia L.) fruit juice on hyperuricemia and the underlying molecular mechanisms were investigated in mouse model of hyperuricemia induced by potassium oxonate using biochemical and high-throughput RNA sequencing analyses.ResultsThe levels of serum uric acid (UA) and xanthine oxidase (XOD) in mice treated with noni fruit juice were significantly decreased, suggesting that the noni fruit juice could alleviate hyperuricemia by inhibiting the XOD activity and reducing the level of serum UA. The contents of both serum creatinine and blood urine nitrogen of the noni fruit juice group were significantly lower than those of the model group, suggesting that noni fruit juice promoted the excretion of UA without causing deleterious effect on the renal functions in mice. The differentially expressed microRNAs involved in the pathogenesis of hyperuricemia in mice were identified by RNA sequencing with their target genes further annotated based on both Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases to explore the metabolic pathways and molecular mechanisms underlying the therapeutic effect on hyperuricemia by noni fruit juice.ConclusionOur study provided strong experimental evidence to support the further investigations of the potential application of noni fruit juice in the treatment of hyperuricemia.

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Section: Discussionmentioning

confidence: 99%

Characterizations of microRNAs involved in the molecular mechanisms underlying the therapeutic effects of noni (Morinda citrifolia L.) fruit juice on hyperuricemia in mice

Liu

Wang

et al. 2023

Front. Nutr.

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“…Since the stem shape of the hydroponic Chinese kale is approximately cylindrical, it was assumed to be an elastic body with transverse isotropy. The constitutive stress-strain relationships were established by Formulas (1) to (8) [23,24].…”

Section: Methodsmentioning

confidence: 99%

“…Hydroponic Chinese kale is one of the clean, short-growth cycles, and high-value leaf vegetables widely planted in the southern China facility enterprise [1][2][3]. Currently, fresh hydroponic Chinese kales are harvested by being grabbed out of the pipeline and root cut using the manual method, which leads to high labor intensity and cost problems [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Finite Element Simulation Parameter Calibration and Verification for Stem Cutting of Hydroponic Chinese Kale

Xia,

Li,

Deng

et al. 2024

Agriculture

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The finite element simulation is a valid way for the rapid development of the root-cutting mechanism for hydroponic Chinese kale. The stem of the hydroponic Chinese kale was simplified as a transverse isotropic elastic body, and axial compression, three-point bending, and shear tests were performed. The ANSYS/LS-DYNA19.2 software was adopted for stem shear simulation, and the regression equation of the maximum simulated shear force was established. The optimized mechanical parameters were determined by minimizing the deviation between the maximum shear force obtained from the simulation and test. The three-dimensional scanning method was employed to establish the geometric model of the hydroponic Chinese kale stem. The cutting finite element simulation model and test platform were constructed. Displacement, deformation, and force measured from simulation and test were compared. Through measurement and simulation calibration, an axial elastic modulus of 6.22 MPa, axial Poisson’s ratio of 0.46, radial elastic modulus of 3.56 MPa, radial Poisson’s ratio of 0.44, radial shear modulus of 0.8 MPa, and a failure strain of 0.08 were determined. During the cutting simulation and test, the resulting maximum displacement deviations of the marking points on the end of the stem were 0.68 mm along the X-axis and 2.83 mm along the Y-axis, while the maximum deviations of the cutting and clamping force were 0.49 N and 0.77 N, respectively. The deformation and force variation laws of the kale stem in the cutting simulation and test process were basically consistent. It showed that the mechanical parameters calibrated by the simulation were accurate and effective, and the stem cutting simulation results with the finite element method were in good agreement with that of the cutting test. The study provided a reference for the rapid optimization design of the root-cutting mechanism for hydroponic Chinese kale harvest.

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“…In addition, there are other, non-canonical pathways for miRNA production. These include so-called “mirtrons”, spliced introns of mRNA, fully Dicer-independent miRNA which is derived from endogenous shRNA processed by Drosha and cleaved by hAgo2 (Human Protein argonaute-2) or m7g (7-methylguanosine)-capped pre-miRNA that can be exported to the cytoplasm without Drosha cleavage [ 58 , 59 , 60 ]. In many cases, multiple miRNAs are transcribed as one long transcript (cluster) that is subsequently cleaved.…”

Section: Nucleic Acids In Rnaimentioning

confidence: 99%

Basic Principles of RNA Interference: Nucleic Acid Types and In Vitro Intracellular Delivery Methods

Isenmann,

Stoddart,

Schmelzeisen

et al. 2023

Micromachines

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Since its discovery in 1989, RNA interference (RNAi) has become a widely used tool for the in vitro downregulation of specific gene expression in molecular biological research. This basically involves a complementary RNA that binds a target sequence to affect its transcription or translation process. Currently, various small RNAs, such as small interfering RNA (siRNA), micro RNA (miRNA), small hairpin RNA (shRNA), and PIWI interacting RNA (piRNA), are available for application on in vitro cell culture, to regulate the cells’ gene expression by mimicking the endogenous RNAi-machinery. In addition, several biochemical, physical, and viral methods have been established to deliver these RNAs into the cell or nucleus. Since each RNA and each delivery method entail different off-target effects, limitations, and compatibilities, it is crucial to understand their basic mode of action. This review is intended to provide an overview of different nucleic acids and delivery methods for planning, interpreting, and troubleshooting of RNAi experiments.

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Research progress on microRNA in gout

Cited by 4 publications

References 101 publications

Characterizations of microRNAs involved in the molecular mechanisms underlying the therapeutic effects of noni (Morinda citrifolia L.) fruit juice on hyperuricemia in mice

Characterizations of microRNAs involved in the molecular mechanisms underlying the therapeutic effects of noni (Morinda citrifolia L.) fruit juice on hyperuricemia in mice

Finite Element Simulation Parameter Calibration and Verification for Stem Cutting of Hydroponic Chinese Kale

Basic Principles of RNA Interference: Nucleic Acid Types and In Vitro Intracellular Delivery Methods

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