Monitoring amphibian populations, especially over fragile ecosystems such as arid and semiarid landscapes, helps to detect demographic trends and ecological risks. During several campaigns carried out between 2013 and 2017 across the Moulouya River Basin and the Oriental Region of Morocco, we identified nine amphibian species from the 11 previously recorded. Amphibian population threats were also detected. In this work, we focused on the amphibians of the Oriental Region of Morocco with the aims to (1) update their distribution providing new records, (2) confirm the prevalence of some vulnerable species in the region, and (3) provide an overview of the ecology and conservation status of each species at a regional level.