Residual toxicity and sublethal effects of fenvalerate on the development and physiology of Spodoptera exigua reared on different hosts

Khan, Muhammad Musa; Hafeez, Muhammad; Siddiqui, Junaid Ali; Ullah, Farman; Shah, Sakhawat; Iftikhar, Ayesha; Rehman, Saad; Rajput, Shahid Ali; Ali, Daoud; Almarzoug, Mohammed H. A.; Sudheer, Surya

doi:10.1016/j.jksus.2021.101593

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…Moreover, different detoxification pathways can be used as biomarkers to assess the effects of a specific insecticide on an organism. Khan et al (2021a) reported many detoxification-related pathways in insects that play key roles in gene regulation during insect host exposure to insecticides. The current study also revealed many detoxification pathways (e.g., metabolic pathways, the insulin signaling pathway, the AMPK signaling pathway, carbon metabolism and peroxisomes, fatty acid metabolism, the metabolism of xenobiotics by cytochrome P450 and the drug metabolism–enzyme pathway) that were significantly differentially regulated due to the exposure of S. invicta to fipronil and beta-cypermethrin.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta

Siddiqui

Luo

Sheikh³

et al. 2022

Front. Physiol.

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Insecticide resistance poses many challenges in insect pest control, particularly in the control of destructive pests such as red imported fire ants (Solenopsis invicta). In recent years, beta-cypermethrin and fipronil have been extensively used to manage invasive ants, but their effects on resistance development in S. invicta are still unknown. To investigate resistance development, S. invicta was collected from populations in five different cities in Guangdong, China. The results showed 105.71- and 2.98-fold higher resistance against fipronil and beta-cypermethrin, respectively, in the Guangzhou population. The enzymatic activities of acetylcholinesterase, carboxylases, and glutathione S-transferases significantly increased with increasing beta-cypermethrin and fipronil concentrations. Transcriptomic analysis revealed 117 differentially expressed genes (DEGs) in the BC-ck vs. BC-30 treatments (39 upregulated and 78 downregulated), 109 DEGs in F-ck vs. F-30 (33 upregulated and 76 downregulated), and 499 DEGs in BC-30 vs. F-30 (312 upregulated and 187 downregulated). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEGs associated with insecticide resistance were significantly enriched in metabolic pathways, the AMPK signaling pathway, the insulin signaling pathway, carbon metabolism, peroxisomes, fatty acid metabolism, drug metabolism enzymes and the metabolism of xenobiotics by cytochrome P450. Furthermore, we found that DEGs important for insecticide detoxification pathways were differentially regulated under both insecticide treatments in S. invicta. Comprehensive transcriptomic data confirmed that detoxification enzymes play a significant role in insecticide detoxification and resistance development in S. invicta in Guangdong Province. Numerous identified insecticide-related genes, GO terms, and KEGG pathways indicated the resistance of S. invicta workers to both insecticides. Importantly, this transcriptome profile variability serves as a starting point for future research on insecticide risk evaluation and the molecular mechanism of insecticide detoxification in invasive red imported fire ants.

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Section: Discussionmentioning

confidence: 99%

“…The evaluation of the side effects of sublethal insecticide doses may indicate adaptations of biological control agents to sublethal toxic stresses. Additionally, this approach might supply valuable information for improving the insect pest control practices using effective insecticides ( Nawaz et al, 2018 ; Khan et al, 2021a ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta

Siddiqui

Luo

Sheikh³

et al. 2022

Front. Physiol.

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“…Insecticide metabolism studies indicated that esterases are involved in resistance mechanisms. Increased accumulation of esterase hydrolytic products in insecticide-resistant insects compared to susceptible gives evidence for the involvement of these enzymes ( Jensen, 2000 ; Silva et al, 2012 ; Khan et al, 2021b ; Siddiqui et al, 2022b ).…”

Section: Resistance In Invasive Insectsmentioning

confidence: 99%

Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies

et al. 2023

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Threatening the global community is a wide variety of potential threats, most notably invasive pest species. Invasive pest species are non-native organisms that humans have either accidentally or intentionally spread to new regions. One of the most effective and first lines of control strategies for controlling pests is the application of insecticides. These toxic chemicals are employed to get rid of pests, but they pose great risks to people, animals, and plants. Pesticides are heavily used in managing invasive pests in the current era. Due to the overuse of synthetic chemicals, numerous invasive species have already developed resistance. The resistance development is the main reason for the failure to manage the invasive species. Developing pesticide resistance management techniques necessitates a thorough understanding of the mechanisms through which insects acquire insecticide resistance. Insects use a variety of behavioral, biochemical, physiological, genetic, and metabolic methods to deal with toxic chemicals, which can lead to resistance through continuous overexpression of detoxifying enzymes. An overabundance of enzymes causes metabolic resistance, detoxifying pesticides and rendering them ineffective against pests. A key factor in the development of metabolic resistance is the amplification of certain metabolic enzymes, specifically esterases, Glutathione S-transferase, Cytochromes p450 monooxygenase, and hydrolyses. Additionally, insect guts offer unique habitats for microbial colonization, and gut bacteria may serve their hosts a variety of useful services. Most importantly, the detoxification of insecticides leads to resistance development. The complete knowledge of invasive pest species and their mechanisms of resistance development could be very helpful in coping with the challenges and effectively developing effective strategies for the control of invasive species. Integrated Pest Management is particularly effective at lowering the risk of chemical and environmental contaminants and the resulting health issues, and it may also offer the most effective ways to control insect pests.

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“…A sublethal concentration causes no apparent or less mortality [ 20 ]. Sublethal concentrations of insecticides are generally considered below the median lethal concentration (LD 50 /LC 50 ) [ 21 , 22 , 23 , 24 ]. In contrast to the majority of evolutionary events, resistance has significant practical and economic implications.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Detoxification Mechanism Assessment of Red Imported Fire Ant (Solenopsis invicta) against Indoxacarb

et al. 2022

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The red imported fire ant (Solenopsis invicta) is one of the deadliest invasive ant species that threatens the world by disrupting biodiversity, important functions within a natural ecosystem, and community structure. They are responsible for huge economic losses in the infested countries every year. Synthetic insecticides, especially indoxacarb, have been broadly used to control S. invicta for many years. However, the biochemical response of S. invicta to indoxacarb remains largely undiscovered. Here, we used the sublethal doses of indoxacarb on the S. invicta collected from the eight different cities of Southern China. The alteration in the transcriptome profile of S. invicta following sublethal dosages of indoxacarb was characterized using high-throughput RNA-seq technology. We created 2 libraries, with 50.93 million and 47.44 million clean reads for indoxacarb treatment and control, respectively. A total of 2018 unigenes were regulated after insecticide treatment. Results indicated that a total of 158 differentially expressed genes (DEGs) were identified in the indoxacarb-treated group, of which 100 were significantly upregulated and 58 were downregulated, mostly belonging to the detoxification enzymes, such as AChE, CarE, and GSTs. Furthermore, results showed that most of these DEGs were found in several KEGG pathways, including steroid biosynthesis, other drug metabolizing enzymes, glycerolipid metabolism, chemical carcinogenesis, drug-metabolizing cytochrome P450, glutathione metabolism, glycerophospholipid metabolism, glycolysis/gluconeogenesis, and metabolism of xenobiotics. Together, these findings indicated that indoxacarb causes significant alteration in the transcriptome profile and signaling pathways of S. invicta, providing a foundation for further molecular inquiry.

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Residual toxicity and sublethal effects of fenvalerate on the development and physiology of Spodoptera exigua reared on different hosts

Cited by 8 publications

References 30 publications

Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta

Transcriptome analysis reveals differential effects of beta-cypermethrin and fipronil insecticides on detoxification mechanisms in Solenopsis invicta

Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies

Comprehensive Detoxification Mechanism Assessment of Red Imported Fire Ant (Solenopsis invicta) against Indoxacarb

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