Residue Determination, Thin-Layer Chromatographic Procedure for Determination of Hydrocortisone Acetate and Alcohol Residues in Milk

Mooney, R. P.; Pasarela, N. R.

doi:10.1021/jf60143a004

Cited by 5 publications

(1 citation statement)

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“…In the case of steroid hormones, which are hydrophobic chemicals, they are extracted using organic solvent followed by clean‐up or concentration procedures. A variety of organic solvents, such as methanol, acetonitrile, ethyl acetate, dichloromethane, n ‐hexane, chloroform and diethyl ether, are used to extract steroid hormones in bovine milk and bovine liver (Mooney and Pasarela ; Simpson ). In this paper, acetonitrile, ethyl acetate and methanol coupled with ultrasonic treatment were selected to extract steroid hormones from bovine milk.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of 22 residual steroid hormones in milk by liquid chromatography–tandem mass spectrometry

Wang

Wei

et al. 2019

Int J of Dairy Tech

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Food safety and consumers’ health are paramount; therefore, it is essential to establish a novel method to determine hormone content in milk. Herein, a novel method was developed that can simultaneously determine 22 residual steroid hormones in milk. To obtain the maximum detection sensitivity, the preparation method of sample, mass spectrometer parameters and liquid chromatography conditions were optimised. The samples were concentrated using Oasis HLB solid‐phase extraction cartridge, followed by quantification via liquid chromatography (C18 column)–tandem mass spectrometer (LC‐MS). Determination of oestrogens and glucocorticoids was conducted in negative mode, whereas androgens and progesterone were in positive multiple reaction monitoring mode. The limit of detection (LOD) and limit of quantification (LOQ) of the target compounds were 0.10–1.20 μg/kg and 0.33–3.96 μg/kg, respectively. The average extraction recoveries of 22 steroid hormones were generally high (82.6–95.3%). The proposed method can be an effective alternative to measure hormonal compounds in milk.

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Section: Resultsmentioning

confidence: 99%

Simultaneous determination of 22 residual steroid hormones in milk by liquid chromatography–tandem mass spectrometry

Wang

Wei

et al. 2019

Int J of Dairy Tech

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show abstract

Corticosteroids

1983

Studies in Analytical Chemistry

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Thin-Layer Chromatography III. A Bibliography of its Use as a Technique in Food Analysis

Brian¹

1969

Canadian Institute of Food Technology Journal

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Residue Determination, Thin-Layer Chromatographic Procedure for Determination of Hydrocortisone Acetate and Alcohol Residues in Milk

Abstract: The financial support of the National Research Council of Canada is gratefully acknowledged. The authors are indebted to D. Murray for operation of the amino acid analyzer. Literature Cited(1) Hall, O., Hereditas 45, 495 (1959).

Cited by 5 publications

References 1 publication

Simultaneous determination of 22 residual steroid hormones in milk by liquid chromatography–tandem mass spectrometry

Simultaneous determination of 22 residual steroid hormones in milk by liquid chromatography–tandem mass spectrometry

Corticosteroids

Thin-Layer Chromatography III. A Bibliography of its Use as a Technique in Food Analysis

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