Resistance to arsenate toxicity in the blue-green alga <i>Synechococcus leopoliensis</i>

Budd, K.; Craig, Susan R.

doi:10.1139/b81-207

Cited by 35 publications

(21 citation statements)

References 15 publications

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“…variabilis was very resistant to the toxic effects of arsenate, as are other cyanobacteria (6,21). Concentrations of arsenate in the 10 to 100 mM range had a toxic effect, but at the lower arsenate concentrations, only cells previously starved for phosphate and then grown with arsenate in the absence of phosphate were particularly sensitive.…”

Section: Resultsmentioning

confidence: 94%

“…Others have reported that arsenate did not compete with phosphate for transport into Synechococcus spp. However, they did not try concentrations of arsenate higher than 100 or 200 ,uM (6,11). Arsenate did inhibit transport of phosphate into A. variabilis (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Physiological studies suggested that arsenate could enter cells, particularly phosphate-starved cells, and it has been shown that arsenate is taken up, although very poorly, by S. leopoliensis (6) …”

Section: Resultsmentioning

confidence: 99%

“…Many cyanobacteria are resistant to concentrations of arsenate that are orders of magnitude greater than those found in natural waters (6,21). In two strains of Synechococcus spp., which are very resistant to arsenate, the analog does not inhibit phosphate transport and is therefore probably not transported by the phosphate transport system (6,11). Synechococcus leopoliensis is more sensitive to arsenite than to arsenate; arsenite interferes with photosynthesis and biosynthesis of amino acids (5).…”

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confidence: 99%

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Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis

Thiel

1988

J Bacteriol

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Cells of the cyanobacterium Anabaena variabiis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition of Pi.Phosphate-starved cells were more sensitive to the toxic effects of arsenate than were unstarved cells, but phosphate could alleviate some of the toxicity. Arsenate was a noncompetitive inhibitor of phosphate transport; however, the apparent Ki values were high, particularly for phosphate-replete cells. Preincubation of phosphate-starved cells with arsenate caused subsequent inhibition of phosphate transport, suggesting that intracellular arsenate inhibited phosphate transport. This effect was not seen in phosphate-replete cells.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis

Thiel

1988

J Bacteriol

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“…However, some microalgae and cyanobacteria, including Chlorella (Maeda et al 1985) and Synechococcus (Budd and Craig 1981), show some resistance to arsenic. Some macroalgae are known to concentrate arsenic to far higher levels than in the surrounding water (Kaise et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Insertional Mutagenesis in a Homologue of a Pi Transporter Gene Confers Arsenate Resistance on Chlamydomonas

Kobayashi

Fujiwara

Shimogawara

et al. 2003

Plant and Cell Physiology

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;An arsenate-resistant mutant AR3 of Chlamydomonas reinhardtii is a recessive mutant generated by random insertional mutagenesis using the ARG7 gene. AR3 shows about 10-fold resistance against arsenate toxicity compared with the wild type. By using a flanking region of an inserted tag as a probe, we cloned the corresponding wild-type allele (PTB1) of a mutated gene, which could completely complement the arsenate-resistance phenotype of AR3. The size of PTB1 cDNA is about 6.0 kb and it encodes a putative protein comprising 1,666 amino acid residues. This protein exhibits significant sequence similarity with the yeast Pho89 protein, which is known to be a Na + /Pi co-transporter, although the PTB1 protein carries an additional Gln-and Gly-rich large hydrophilic region in the middle of its primary structure. Analyses of arsenic accumulation and release revealed that PTB1-disrupted cells show arsenate resistance due to low arsenate uptake. These results suggest that the PTB1 protein is a factor involved in arsenate (or Pi) uptake. Kinetics of Pi uptake revealed that the activity of high-affinity Pi transport component in AR3 is more activated than that in the wild type.

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Environmental factors relating to arsenic accumulation by Dunaliella sp

Yamaoka¹,

Takimura²,

Fuse³

1988

Applied Organom Chemis

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The accumulation of arsenic by Dunaliella sp. was examined by using a solution containing arsenic only as a first approach to the study of arsenic recovery by aqueous systems. The accumulation of arsenic by Dunaliella sp. was rapid, with equilibrium established in 8 h with respect to arsenic partitioning between dissolved and particulate phase. The optimum accumulation was at pH 8.2, NaCl 20 g dm-3, illumination 5000-10000 lux and temperature 22 O C. Increased phosphate coucentration significantly decreased the uptake of arsenic in the culture. These results suggested that accumulation of arsenic by Dunaliella sp. depended upon biological activity.

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Resistance to arsenate toxicity in the blue-green alga Synechococcus leopoliensis

Cited by 35 publications

References 15 publications

Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis

Phosphate transport and arsenate resistance in the cyanobacterium Anabaena variabilis

Insertional Mutagenesis in a Homologue of a Pi Transporter Gene Confers Arsenate Resistance on Chlamydomonas

Environmental factors relating to arsenic accumulation by Dunaliella sp

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