Resistance to benzalkonium chloride, peracetic acid and nisin during formation of mature biofilms by Listeria monocytogenes

Ibusquiza, Paula Saá; Herrera, Juan José Rodríguez; Cabo, Marta López

doi:10.1016/j.fm.2010.09.014

Cited by 106 publications

(82 citation statements)

References 52 publications

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“…Similar results were observed by Lee et al (2016), indicating that PAA (0.5%) was able to inactivate the biofilm of L. monocytogenes formed on stainless steel. Our results are also in agreement with those reported by Ibusquiza, Herrera and Cabo (2011), who observed that PAA treatment for 10 min was effective in eliminating L. monocytogenes biofilms. The authors considered that the high oxidizing capacity and low molecular size of PAA are advantages for biofilm penetration.…”

Section: Resultssupporting

confidence: 93%

“…PAA has a broad spectrum and is a strong oxidizing agent that is decomposed into safe waste products (Van der Veen, Abee, 2011). Several studies have demonstrated that PAA was efficient in removing adhered cells of L. monocytogenes (Belessi et al, 2011;Ibusquiza, Herrera, Cabo, 2011;Stoporth et al, 2002). Lee et al (2016) also found that PAA at 0.5% was able to inactivate biofilms formed by a L. monocytogenes strain isolated from brine on stainless steel, but was not effective for removing their adherent cells on polystyrene microplates.…”

Section: Introductionmentioning

confidence: 99%

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Effect of peracetic acid on biofilms formed by Listeria monocytogenes strains isolated from a Brazilian cheese processing plant

Lee

Barancelli

Corassin

et al. 2017

Braz. J. Pharm. Sci.

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This study aimed to investigate the effect of peracetic acid (PAA, 0.5%) on adherent cells of three strains of Listeria monocytogenes strains belonging to serotypes 4b and 1/2b that had been previously isolated from the environment of a Brazilian cheese plant. The assays were conducted using polystyrene microplates and stainless steel coupons and the adhered cells were treated with PAA for 60, 120 and 180 s. On stainless steel, biofilms were partially inactivated by PAA after 60 s and almost 100% of the cells were damaged within 180 s using epifluorescence microscopy with LIVE/DEAD® staining. On polystyrene microplates, PAA decreased (P<0.05) biofilm biomass produced by the three L. monocytogenes isolates at 60 s, when compared with controls (no PAA treatment). However, PAA did not completely eliminate L. monocytogenes cells on polystyrene microplates (decreasing 1.8-2.5 log cycles after treatment with PAA for 180 s). The correct concentration and contact time of PAA is critical for eliminating biofilms formed by L. monocytogenes on stainless steel surfaces, although further studies are needed for defining efficient PAA treatments to remove adherent cells of this pathogen on plastic polymers.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Effect of peracetic acid on biofilms formed by Listeria monocytogenes strains isolated from a Brazilian cheese processing plant

Lee

Barancelli

Corassin

et al. 2017

Braz. J. Pharm. Sci.

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“…Artificial P. aeruginosa biofilms resisted treatment with various biocidal agents including peracetic acid, compared with their planktonic counterparts [150][151][152] . Biofilms composed of E. coli [152,153] , S. aureus [152,154,155] , Mycobacterium fortuitum [156] or Listeria monocytogenes [157] also resisted treatment with diverse biocides compared with planktonic cells. Bacteria in mature (old) biofilms were more resistant to killing than those in young biofilms [153,158,159] .…”

Section: Resistance Of Biofilm Bacteriamentioning

confidence: 99%

Is peracetic acid suitable for the cleaning step of reprocessing flexible endoscopes?

Kampf

Fliss

Martiny

2014

WJGE

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The bioburden (blood, protein, pathogens and biofilm) on flexible endoscopes after use is often high and its removal is essential to allow effective disinfection, especially in the case of peracetic acid-based disinfectants, which are easily inactivated by organic material. Cleaning processes using conventional cleaners remove a variable but often sufficient amount of the bioburden. Some formulations based on peracetic acid are recommended by manufacturers for the cleaning step. We performed a systematic literature search and reviewed the available evidence to clarify the suitability of peracetic acid-based formulations for cleaning flexible endoscopes. A total of 243 studies were evaluated. No studies have yet demonstrated that peracetic acidbased cleaners are as effective as conventional cleaners. Some peracetic acid-based formulations have demonstrated some biofilm-cleaning effects and no biofilmfixation potential, while others have a limited cleaning effect and a clear biofilm-fixation potential. All published data demonstrated a limited blood cleaning effect and a substantial blood and nerve tissue fixation potential of peracetic acid. No evidence-based guidelines on reprocessing flexible endoscopes currently recommend using cleaners containing peracetic acid, but some guidelines clearly recommend not using them because of their fixation potential. Evidence from some outbreaks, especially those involving highly multidrug-resistant gram-negative pathogens, indicated that disinfection using peracetic acid may be insufficient if the preceding cleaning step is not performed adequately. Based on this review we conclude that peracetic acid-based formulations should not be used for cleaning flexible endoscopes. Key words: Peracetic acid; Cleaning; Flexible endoscope; Biofilm; Resistance; Bioburden; Blood; Disinfection; Reprocessing Core tip: Some formulations based on peracetic acid (PAA) are recommended by manufacturers for cleaning flexible endoscopes. We reviewed 243 studies to analyse the evidence for this recommendation. No study demonstrated that PAA-based cleaners were as effective as conventional cleaners, and some PAA-based formulations had clear biofilm-fixation potential. Dried blood and nerve tissue were substantially fixed by PAA. Some outbreaks, especially of highly multidrug-resistant gram-negative pathogens, indicated that insufficient cleaning could not be compensated for by using PAA in the disinfection step. PAA-based formulations should not be used for cleaning flexible endoscopes.

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“…Benzalkonium chloride (BAC) is considered one of the most used QACs due to its action upon bacterial membranes altering their structural integrity (Gerba 2015). Nevertheless, it has been extensively demonstrated that biofilms exhibit higher resistance/tolerance to BAC compared to planktonic cells both in Gram-positives such as L. monocytogenes (Saá Ibusquiza et al 2011;Nakamura et al 2013) and in Gram-negatives such as Escherichia coli (Houari & Di Martino 2007) or Pseudomonas sp. (Giaouris et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

et al. 2016

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Resistance to benzalkonium chloride, peracetic acid and nisin during formation of mature biofilms by Listeria monocytogenes

Cited by 106 publications

References 52 publications

Effect of peracetic acid on biofilms formed by Listeria monocytogenes strains isolated from a Brazilian cheese processing plant

Effect of peracetic acid on biofilms formed by Listeria monocytogenes strains isolated from a Brazilian cheese processing plant

Is peracetic acid suitable for the cleaning step of reprocessing flexible endoscopes?

Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

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