Resistance to stem rust and a molecular marker analysis of Sr genes in spring bread wheat introgression lines

Abstract

128 introgression lines of spring bread wheat developed by Agricultural Research Institute of the South-East Region and 11 cultivars cultivated in the Volga Region were analyzed for resistance to the Ug99 race group in Kenya (KARI). 10 immune lines (infection type 0) and 16 lines with moderate resistance (infection type MR) were identified. Cultivars and part of the lines (58 introgression lines) were assessed for resistance to Lysogorsk and Omsk stem rust pathogen populations and to stem rust isolates PgtZ1(TKSTF) and PgtF18.6 (TKSTF+Sr33) and also analyzed for the presence of the known Sr resistance genes (Sr22, Sr25, Sr26, Sr31, Sr35, Sr36, Sr38, Sr39) using molecular markers. The Sr31/Lr26, Sr25/Lr19, Sr22, Sr35 and Sr38/Lr37 genes were identified in the introgression lines. All lines carrying Sr31/Lr26 and Sr22 were resistant to all local pathogen populations taken into analysis. The Sr26, Sr36 and Sr39 genes were not detected in the wheat lines analyzed.